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              <name>Title</name>
              <description>A name given to the resource</description>
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                  <text>Coronavirus</text>
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                  <text>Dominio científico: Coronavirus</text>
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                <text>Generation of a Nebulizable CDR-Modified MERS-CoV Neutralizing Human Antibody</text>
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                <text>Sang-il Kim, Sujeong Kim, Jin-Hee Kim, Soyoung Chang, Jung Min Shim, Jongwha Jin, Chungsu Lim, Songyi Baek, Ji-Young MIN, Wan Beom Park, Myoung-don Oh, Seungtaek Kim, Junho Chung</text>
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            <description>An account of the resource</description>
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                <text>Middle East respiratory syndrome coronavirus (MERS-CoV) induces severe aggravating respiratory failure in infected patients, frequently resulting in mechanical ventilation. As limited therapeutic antibody is accumulated in lung tissue following systemic administration, inhalation is newly recognized as an alternative, possibly better, route of therapeutic antibody for pulmonary diseases. The nebulization process, however, generates diverse physiological stresses, and thus, the therapeutic antibody must be resistant to these stresses, remain stable, and form minimal aggregates. We first isolated a MERS-CoV neutralizing antibody that is reactive to the receptor-binding domain (RBD) of spike (S) glycoprotein. To increase stability, we introduced mutations into the complementarity-determining regions (CDRs) of the antibody. In the HCDRs (excluding HCDR3) in this clone, two hydrophobic residues were replaced with Glu, two residues were replaced with Asp, and four residues were replaced with positively charged amino acids. In LCDRs, only two Leu residues were replaced with Val. These modifications successfully generated a clone with significantly greater stability and equivalent reactivity and neutralizing activity following nebulization compared to the original clone. In summary, we generated a MERS-CoV neutralizing human antibody that is reactive to recombinant MERS-CoV S RBD protein for delivery via a pulmonary route by introducing stabilizing mutations into five CDRs.</text>
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            <description>A point or period of time associated with an event in the lifecycle of the resource</description>
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                <text>2019</text>
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          <element elementId="49">
            <name>Subject</name>
            <description>The topic of the resource</description>
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              <elementText elementTextId="10785">
                <text>MERS-CoV, Aerosol delivery, Nebulizer, neutralizing antibody, antibody engineering, Pulmonary disease, complementarity determining regions</text>
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            <name>Identifier</name>
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              <elementText elementTextId="10786">
                <text>DOI: 10.3390/ijms20205073</text>
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            <name>Source</name>
            <description>A related resource from which the described resource is derived</description>
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              <elementText elementTextId="10787">
                <text>International Journal of Molecular Sciences</text>
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          <element elementId="45">
            <name>Publisher</name>
            <description>An entity responsible for making the resource available</description>
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                <text>MDPI AG</text>
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            <description>The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant</description>
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                <text>Biology (General), Chemistry</text>
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            <description>A language of the resource</description>
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                <text>EN</text>
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              <name>Title</name>
              <description>A name given to the resource</description>
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                  <text>Coronavirus</text>
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              <name>Description</name>
              <description>An account of the resource</description>
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                  <text>Dominio científico: Coronavirus</text>
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            </element>
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      <name>Text</name>
      <description>A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.</description>
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        <name>Dublin Core</name>
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                <text>Generation of human antibody fragments recognizing distinct epitopes of the nucleocapsid (N) SARS-CoV protein using a phage display approach</text>
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            <name>Creator</name>
            <description>An entity primarily responsible for making the resource</description>
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              <elementText elementTextId="21263">
                <text>Grasso Felicia, Carattoli Alessandra, Zamboni Silvia, Ascione Alessandro, Di Bonito Paola, Flego Michela, Cassone Antonio, Cianfriglia Maurizio</text>
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            <name>Description</name>
            <description>An account of the resource</description>
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                <text>Abstract Background Severe acute respiratory syndrome (SARS)-CoV is a newly emerging virus that causes SARS with high mortality rate in infected people. Successful control of the global SARS epidemic will require rapid and sensitive diagnostic tests to monitor its spread, as well as, the development of vaccines and new antiviral compounds including neutralizing antibodies that effectively prevent or treat this disease. Methods The human synthetic single-chain fragment variable (scFv) ETH-2 phage antibody library was used for the isolation of scFvs against the nucleocapsid (N) protein of SARS-CoV using a bio panning-based strategy. The selected scFvs were characterized under genetics-molecular aspects and for SARS-CoV N protein detection in ELISA, western blotting and immunocytochemistry. Results Human scFv antibodies to N protein of SARS-CoV can be easily isolated by selecting the ETH-2 phage library on immunotubes coated with antigen. These in vitro selected human scFvs specifically recognize in ELISA and western blotting studies distinct epitopes in N protein domains and detect in immunohistochemistry investigations SARS-CoV particles in infected Vero cells. Conclusion The human scFv antibodies isolated and described in this study represent useful reagents for rapid detection of N SARS-CoV protein and SARS virus particles in infected target cells.</text>
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            <name>Date</name>
            <description>A point or period of time associated with an event in the lifecycle of the resource</description>
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              <elementText elementTextId="21265">
                <text>2005</text>
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          <element elementId="43">
            <name>Identifier</name>
            <description>An unambiguous reference to the resource within a given context</description>
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              <elementText elementTextId="21266">
                <text>DOI: 10.1186/1471-2334-5-73</text>
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          <element elementId="48">
            <name>Source</name>
            <description>A related resource from which the described resource is derived</description>
            <elementTextContainer>
              <elementText elementTextId="21267">
                <text>BMC Infectious Diseases</text>
              </elementText>
            </elementTextContainer>
          </element>
          <element elementId="45">
            <name>Publisher</name>
            <description>An entity responsible for making the resource available</description>
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              <elementText elementTextId="21268">
                <text>BMC</text>
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            <name>Coverage</name>
            <description>The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant</description>
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              <elementText elementTextId="21269">
                <text>Infectious and parasitic diseases</text>
              </elementText>
            </elementTextContainer>
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            <description>A language of the resource</description>
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                <text>EN</text>
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              <name>Title</name>
              <description>A name given to the resource</description>
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                <elementText elementTextId="1">
                  <text>Coronavirus</text>
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              <name>Description</name>
              <description>An account of the resource</description>
              <elementTextContainer>
                <elementText elementTextId="2">
                  <text>Dominio científico: Coronavirus</text>
                </elementText>
              </elementTextContainer>
            </element>
          </elementContainer>
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      <name>Text</name>
      <description>A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.</description>
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          <element elementId="50">
            <name>Title</name>
            <description>A name given to the resource</description>
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              <elementText elementTextId="29834">
                <text>Generational Differences in Perceptions of Food Health/Risk and Attitudes toward Organic Food and Game Meat: The Case of the COVID-19 Crisis in China</text>
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          <element elementId="39">
            <name>Creator</name>
            <description>An entity primarily responsible for making the resource</description>
            <elementTextContainer>
              <elementText elementTextId="29835">
                <text>Hong Zhu, Liman Huang, Jun  (Justin) Li, Xiaoru Xie</text>
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          <element elementId="41">
            <name>Description</name>
            <description>An account of the resource</description>
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              <elementText elementTextId="29836">
                <text>In December 2019, a novel laboratory-confirmed coronavirus (2019-nCoV) infection, which has caused clusters of severe illnesses, was first reported in Wuhan, the capital of Hubei province, China. This foodborne illness, which reportedly most likely originated in a seafood market where wild animals are sold illegally, has transmitted among humans through close contact, across the world. The aim of this study is to explore health/risk perceptions of and attitudes toward healthy/risky food in the immediate context of food crisis. More specifically, by using the data collected from 1008 respondents in January 2020, the time when China was hit hard by the “Corona Virus Disease 2019” (COVID-19), this study investigates the overall and different generational respondents’ health/risk perceptions of and attitudes toward organic food and game meat. The results reveal that, firstly, based on their food health and risk perceptions of healthy and risky food, the respondents’ general attitudes are positive toward organic food but relatively negative toward game meat. Secondly, older generations have a more positive attitude and are more committed to organic food. Younger generations’ attitude toward game meat is more negative whereas older generations attach more importance to it because of its nutritional and medicinal values. In addition, this research also indicates that the COVID-19 crisis influences the respondents’ perceptions of and attitudes toward organic food and game meat consumption. However, the likelihood of its impact on older generations’ future change in diets is smaller, which implies that older generations’ food beliefs are more stable.</text>
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            <description>A point or period of time associated with an event in the lifecycle of the resource</description>
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                <text>2020</text>
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            <name>Subject</name>
            <description>The topic of the resource</description>
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              <elementText elementTextId="29838">
                <text>Attitude, China, game meat, organic food, health risk perception, food safety crisis</text>
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            <name>Identifier</name>
            <description>An unambiguous reference to the resource within a given context</description>
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              <elementText elementTextId="29839">
                <text>DOI: 10.3390/ijerph17093148</text>
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            <name>Source</name>
            <description>A related resource from which the described resource is derived</description>
            <elementTextContainer>
              <elementText elementTextId="29840">
                <text>International Journal of Environmental Research and Public Health</text>
              </elementText>
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            <name>Publisher</name>
            <description>An entity responsible for making the resource available</description>
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              <elementText elementTextId="29841">
                <text>MDPI AG</text>
              </elementText>
            </elementTextContainer>
          </element>
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            <name>Coverage</name>
            <description>The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant</description>
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              <elementText elementTextId="29842">
                <text>Medicine</text>
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              <name>Title</name>
              <description>A name given to the resource</description>
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                  <text>Agricultura sostenible</text>
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            <element elementId="41">
              <name>Description</name>
              <description>An account of the resource</description>
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                <elementText elementTextId="88122">
                  <text>Dominio científico: Agricultura sostenible</text>
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            <name>Title</name>
            <description>A name given to the resource</description>
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              <elementText elementTextId="151935">
                <text>Generational succession in family farming: lessons from two rural communities</text>
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          <element elementId="39">
            <name>Creator</name>
            <description>An entity primarily responsible for making the resource</description>
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              <elementText elementTextId="151936">
                <text>Manoel Adir Kischener, Norma Kiyota, Miguel Angelo Perondi</text>
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            <description>An account of the resource</description>
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                <text>The new pattern of modernization of agriculture and rural, in force in most rural areas of Brazil, is also present in the Southwest of Paraná. Thus, considering the marks left by this process, this research tried to see what were the factors that interfere in generational succession and began to influence the family farm's social reproduction strategy. For that, from a qualitative approach, this study sought to compare two rural communities by establishing 25 interviews each and two focus group sessions. The results of this study, it was noticed that the factors that favor the permanence of young people in rural areas were mainly: income, living in society and the inclusion of life projects of the children in the family's social reproduction strategies.</text>
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            <description>A point or period of time associated with an event in the lifecycle of the resource</description>
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                <text>2015</text>
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          <element elementId="49">
            <name>Subject</name>
            <description>The topic of the resource</description>
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                <text>Agricultura Familiar, História da agricultura, Sudoeste do Paraná, sucessão geracional</text>
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            <name>Source</name>
            <description>A related resource from which the described resource is derived</description>
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                <text>Mundo Agrario</text>
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            <name>Publisher</name>
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                <text>Universidad Nacional de La Plata</text>
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            <description>The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant</description>
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                <text>Social history and conditions. Social problems. Social reform, Sociology (General)</text>
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                <text>&lt;a href="http://www.mundoagrario.unlp.edu.ar/article/view/5471" target="_blank" rel="noreferrer noopener"&gt;http://www.mundoagrario.unlp.edu.ar/article/view/5471&lt;/a&gt;</text>
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                  <text>Dominio científico: Agricultura sostenible</text>
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                <text>http://dx.doi.org/10.1590/S0104-026X2004000100011  O texto examina as formas de inserção das mulheres na agricultura familiar, procurando explicar a seletividade de gênero do processo migratório. Primeiramente, aborda a distribuição da população, por sexo e por grupos de idade, em diversas regiões do Estado do Rio Grande do Sul, caracterizando o maior índice de emigração de mulheres jovens do que dos demais grupos etários e de sexo. Depois, trata da divisão do trabalho por sexo e idade, dos efeitos da modernização sobre o trabalho agrícola, da inserção dos jovens no trabalho da unidade produtiva familiar, das atividades fora da agricultura e dos procedimentos utilizados pelos produtores agrícolas para a transmissão da propriedade rural para os filhos. Finalmente, discute o possível efeito do acesso das mulheres rurais à Previdência Rural sobre suas perspectivas de permanecer ou não na atividade agrícola.</text>
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                <text>El cambio climático es un problema de toda la humanidad; sin embargo, hay inequidad de género en los impactos del cambio climático, donde las mujeres son más impactadas. En el 2010, la Unión Internacional para la Conservación de la Naturaleza (UICN) desarrolló una metodología para elaborar Planes de Acción de Género y Cambio Climático (PAGcc) con el objetivo de promover un enfoque integral y multisectorial ante el cambio climático. El proceso de un PAGcc incluye: 1) Inventario de información; 2) Nivelar el terreno; 3) Tomar en cuenta la diversidad de voces; 4) Priorizar; y 5) Implementación/acción. A la fecha, esta metodología ha sido utilizada por 20 gobiernos nacionales y en tres procesos regionales. La metodología desarrollada por la UICN para elaborar los PAGcc presenta una forma innovadora para transversalizar la perspectiva de género. Se basa en el fortalecimiento de capacidades de los grupos de interés para construir soluciones apropiadas, no convencionales, concretas, prácticas e innovadoras. Un PAGcc se centra en sectores claves identificados por cada país y reconoce la igualdad de género como un motor transformador a nivel nacional.</text>
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                <text>2019</text>
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                <text>Adaptación, UICN, política climática género-responsiva</text>
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                <text>Leonardo Güiza Suárez, Cristhian David Rodríguez Barajas, Blanca Oliva Ríos Osorio, Sara Sofía Moreno Gallo</text>
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                <text>La población rural femenina ha sido vulnerable a los impactos de la desigualdad social y el conflicto armado. Por esta razón, ante condiciones de discriminación, pobreza y violencia, algunas mujeres rurales han encontrado alternativas de cohesión comunitaria a partir de la conservación ambiental, las cuales se han vuelto una forma de mitigar y hacerle frente a esas dificultades. Este artículo muestra los resultados de un estudio de caso llevado a cabo en Vergara, Cundinamarca (Colombia). El objetivo es analizar las acciones de participación comunitaria implementadas por los miembros de la vereda Llano Grande, con el propósito de identificar las potencialidades, dificultades y lecciones aprendidas en torno a la gobernanza ambiental y al empoderamiento comunitario. Se hizo uso de entrevistas a profundidad, encuestas semiestructuradas, observación participante y grupos focales, mediante un modelo de Investigación-Acción Participativa (IAP), para definir estrategias de fortalecimiento local, igualdad de género y gobernanza ambiental en un contexto rural de posconflicto. Se sostiene que la participación comunitaria ambiental ha sido un instrumento para el empoderamiento de las mujeres rurales en dicho contexto y, al mismo tiempo, contribuye positivamente a la consolidación de buenas prácticas de gobernanza ambiental en una perspectiva de género.</text>
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                <text>Conservación ambiental, Gênero, Vergara, mujeres rurales, posconflicto</text>
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                <text>Revista Estudios Socio-Jurídicos</text>
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                <text>Universidad del Rosario</text>
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                <text>Law in general. Comparative and uniform law. Jurisprudence, Law</text>
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                <text>Género y estrategias locales de adaptación ante la variabilidad climática en San Andrés Hueyacatitla, Puebla, México</text>
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                <text>Rosalba del Pilar González Suárez, Beatriz Martínez Corona, María Esther Méndez Cadena, Andrés Pérez Magaña, Verónica Gutiérrez Villalpando</text>
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            <description>An account of the resource</description>
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                <text>Gran parte de las superficies forestales en México son manejadas por comunidades agrarias y ejidos, los cuales cuentan también con territorio destinado a la producción agrícola. Se realizó un estudio de caso en el ejido de San Andrés Hueyacatitla, municipio de San Salvador El Verde, estado de Puebla, donde las estrategias de reproducción de los grupos domésticos ejidales se centran en el manejo del bosque, las actividades agrícolas, así como el trabajo reproductivo y de cuidado. El objetivo fue conocer las afectaciones y estrategias de adaptación ante el cambio climático diferenciadas por género. Para la realización del estudio se tomó una muestra de 80 hombres y mujeres, integrantes de grupos domésticos con derechos ejidales. Se aplicó una encuesta y entrevistas semiestructuradas a informantes clave y se realizó observación participante. Se identificaron las afectaciones que los hombres y mujeres participantes en el estudio reconocen y experimentan de forma diferencial, las cuales son asociadas con la variabilidad del clima o cambio climático. Asimismo se detectó el desarrollo de estrategias de adaptación de acuerdo con sus asignaciones genéricas, conocimientos tradicionales y prácticas. Se encontró que los programas gubernamentales de conservación del bosque favorecen el desarrollo de estrategias de adaptación en los grupos domésticos ejidales para el manejo del bosque, lo cual sin embargo no disminuye plenamente la vulnerabilidad socioambiental en la zona de estudio.</text>
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                <text>2019</text>
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                <text>10.31840/sya.v0i21.2042</text>
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                <text>Sociedad y Ambiente</text>
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            <name>Publisher</name>
            <description>An entity responsible for making the resource available</description>
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              <elementText elementTextId="199555">
                <text>El Colegio de la Frontera Sur</text>
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            <name>Coverage</name>
            <description>The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant</description>
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                <text>Environmental sciences</text>
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                <text>&lt;a href="https://revistas.ecosur.mx/sociedadyambiente/index.php/sya/article/view/2042" target="_blank" rel="noreferrer noopener"&gt;https://revistas.ecosur.mx/sociedadyambiente/index.php/sya/article/view/2042&lt;/a&gt;</text>
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            <element elementId="50">
              <name>Title</name>
              <description>A name given to the resource</description>
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                  <text>Agricultura sostenible</text>
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              <name>Description</name>
              <description>An account of the resource</description>
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                  <text>Dominio científico: Agricultura sostenible</text>
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      <name>Text</name>
      <description>A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.</description>
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                <text>Género y etnia en el devenir del maíz nativo del pueblo matlatzinca</text>
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              <elementText elementTextId="191590">
                <text>Ana Gabriela Rincón Rubio, Ivonne Vizcarra Bordi</text>
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          <element elementId="41">
            <name>Description</name>
            <description>An account of the resource</description>
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              <elementText elementTextId="191591">
                <text>El propósito de este trabajo es conocer las actitudes, valores sociales y tácticas actuales que mujeres y hombres matlatzincas recrean en su vida cotidiana frente al dilema de seguir produciendo maíz nativo como parte de su seguridad alimentaria, al transitar hacia modos de vida urbanos, o bien una combinación de ambos. Es un estudio cualitativo basado en la etnografía feminista que busca la comprensión de la interseccionalidad género-etnia en la producción de maíz nativo. Como técnica de investigación se realizaron veintidós entrevistas semiestructuradas, aplicadas a siete hombres y quince mujeres, miembros de la comunidad de San Francisco Oxtotilpan, Estado de México, que participan en actividades agrícolas relacionadas con los maíces nativos. Los resultados agrupan tres preocupaciones: variabilidad climática, perspectivas de mejora de la situación socioeconómica y arribo de nuevos paquetes biotecnológicos. Asimismo, se discuten las perspectivas sobre el futuro del maíz nativo. Se concluye que para el pueblo matlatzinca el maíz nativo es eje de identidad, y a la vez un recurso de control económico y social, que para ser preservado requiere que los principios de igualdad de género y etnia permanezcan.</text>
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            <name>Date</name>
            <description>A point or period of time associated with an event in the lifecycle of the resource</description>
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              <elementText elementTextId="191592">
                <text>2017</text>
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            <name>Identifier</name>
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                <text>10.31840/sya.v2017i13.1756</text>
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                <text>Sociedad y Ambiente</text>
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          <element elementId="45">
            <name>Publisher</name>
            <description>An entity responsible for making the resource available</description>
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              <elementText elementTextId="191595">
                <text>El Colegio de la Frontera Sur</text>
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          <element elementId="38">
            <name>Coverage</name>
            <description>The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant</description>
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              <elementText elementTextId="191596">
                <text>Environmental sciences</text>
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            <name>Relation</name>
            <description>A related resource</description>
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              <elementText elementTextId="191597">
                <text>&lt;a href="https://revistas.ecosur.mx/sociedadyambiente/index.php/sya/article/view/1756" target="_blank" rel="noreferrer noopener"&gt;https://revistas.ecosur.mx/sociedadyambiente/index.php/sya/article/view/1756&lt;/a&gt;</text>
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