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                  <text>Coronavirus</text>
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                  <text>Dominio científico: Coronavirus</text>
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                <text>Campi di esperienza o campo dell’esperienza?</text>
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                <text>Danilo Russo</text>
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            <description>An account of the resource</description>
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                <text>Oggetto di questo articolo sono i campi d’esperienza, che già dal 1991, e ancora nelle “Indicazioni nazionali…”, articolano in forma originale l’offerta di saperi della scuola dell’infanzia italiana. Si vorrebbe mostrare che le Indicazioni sono l’esito provvisorio di una tendenza lunga e potente a didatticizzare la scuola dell’infanzia, a conformarla alle logiche valutative della scuola che segue. I campi d’esperienza, coi loro 30 anni di vita e la loro centralità, registrano in modo esemplare il procedere di questa tendenza, leggibile nelle successive declinazioni, soprattutto operative, che essi hanno via via ricevuto in testi ministeriali comparabili, fino alle Indicazioni in vigore. La provocazione linguistica del titolo allude alla torsione cui il concetto dei campi d’esperienza è sottoposto, alle aporie cui essa conduce; a un modo diverso e possibile, infine, di pensarlo e di usarlo. Chiudono, a titolo di post scriptum reso urgente dalle circostanze, alcune considerazioni ancora acerbe su come il covid-19, chiudendo le scuole come luogo fisico, strappi il velo dal quadro, e costringa problematicamente tutti a ripensare le proprie opzioni.</text>
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                <text>2020</text>
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                <text>indeterminazione, ambiguità, esperienza/condotte, gioco/curricolo</text>
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                <text>10.5007/1980-4512.2020v22n41p90</text>
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            <description>A related resource from which the described resource is derived</description>
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                <text>Epidemiology and Health</text>
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            <name>Publisher</name>
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                <text>Korean Society of Epidemiology</text>
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            <description>The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant</description>
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                <text>Education</text>
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                  <text>Coronavirus</text>
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                  <text>Dominio científico: Coronavirus</text>
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      <name>Text</name>
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                <text>Isolation in HRT-18 cells and molecular analysis of a BCoV strain from diarrheic feces of naturally infected calves</text>
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            <name>Creator</name>
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                <text>Danilo Tancler Stipp, Aline Fernandes Barry, Alice Fernandes Alfieri, Lívia Bodnar, Amauri Alcindo Alfieri</text>
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                <text>Bovine coronavirus (BCoV) may cause acute diarrhea in newborn calves, leading to significant economic losses for cattle farmers. There are several diagnostic techniques used to detect BCoV in calf fecal samples, but virus isolation still has advantages for antigenic and genomic characterization. This study describes the isolation in HRT-18 cells and molecular characterization of Brazilian BCoV wild-type strains. Three fecal samples from diarrheic 30 day-old calves were inoculated in HRT-18 cell monolayers, which were then evaluated for HA titers and tested using semi-nested PCR followed by RFLP and sequencing. Two samples were successfully isolated and presented HA titers of 16 and 32 units per 25 mL. The results were confirmed using semi-nested PCR and RFLP. Molecular analyses identified a cell culture-adapted strain and a wild-type strain that were genetically similar (99%) to each other, but more distinct than BCoV strains circulating in other countries, even in the conserved N gene.O coronavírus bovino (BCoV) pode causar diarreia aguda em bezerros recém-nascidos, ocasionando consideráveis perdas econômicas para a pecuária bovina. Várias técnicas de diagnóstico podem ser empregadas na detecção do BCoV a partir de amostras fecais de bezerros. Porém, o isolamento do BCoV em cultivo celular apresenta a vantagem de possibilitar a caracterização antigênica e molecular da estirpe viral. O presente estudo descreve o isolamento em células HRT-18, e a caracterização molecular de estirpes brasileiras do BCoV. Três amostras de fezes diarreicas de bezerros com 30 dias de idade foram inoculadas em culturas de células HRT-18. Os isolados foram avaliados por hemaglutinação (HA) e por uma semi-nested PCR seguida de RFLP e sequenciamento. Duas amostras foram isoladas e a confirmação foi verificada na semi-nested PCR e também RFLP. Na HA os títulos foram de 16 e 32 unidades por 25 mL. Análises moleculares identificam a estirpe adaptada em cultura celular e uma estirpe selvagem, como estirpes de BCoV semelhantes (99%) entre si, mas distintas das circulantes em outros países, mesmo em um gene de uma proteína conservada (gene N).</text>
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            <name>Date</name>
            <description>A point or period of time associated with an event in the lifecycle of the resource</description>
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                <text>2009</text>
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            <name>Subject</name>
            <description>The topic of the resource</description>
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                <text>cattle, diarrhea, Bovine coronavirus, Cell culture, molecular analyses</text>
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            <name>Identifier</name>
            <description>An unambiguous reference to the resource within a given context</description>
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                <text>DOI: 10.1590/S1516-89132009000700007</text>
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            <description>A related resource from which the described resource is derived</description>
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                <text>Brazilian Archives of Biology and Technology</text>
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            <name>Publisher</name>
            <description>An entity responsible for making the resource available</description>
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                <text>Instituto de Tecnologia do Paraná (Tecpar)</text>
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            <description>The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant</description>
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                <text>Biotechnology</text>
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            <description>A language of the resource</description>
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                <text>EN</text>
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              <description>A name given to the resource</description>
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                  <text>Agricultura sostenible</text>
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              <description>An account of the resource</description>
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                  <text>Dominio científico: Agricultura sostenible</text>
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      <name>Text</name>
      <description>A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.</description>
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            <name>Title</name>
            <description>A name given to the resource</description>
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                <text>Incorporación de trigo en la rotación agrícola: una herramienta para potenciar el funcionamiento del agroecosistema</text>
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            <name>Creator</name>
            <description>An entity primarily responsible for making the resource</description>
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                <text>Dannae Lilia Serri, José Meriles, Cinthia Conforto, Carolina Pérez Brandan, Silvina Pastor, Betiana Grümberg, Celina Luna, Claudio Lorenzon, Juan Arce, Hugo Marelli, Silvina Vargas Gil</text>
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            <description>An account of the resource</description>
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                <text>Argentina es uno de los principales productores y exportadores de granos a nivel mundial. En particular, la región Pampeana es el área de mayor actividad agrícola centrando su producción en el cultivo de soja, seguido del maíz y trigo en menor proporción. En los últimos años, el sistema basado en la producción continua de un cultivo ha generado un impacto negativo sobre las propiedades del suelo. Históricamente, prácticas conservacionistas como la rotación de cultivos y labranza cero, se han propuesto como alternativas al monocultivo y remoción de residuos de cosecha. De esta manera, la diversificación del sistema (inclusión de gramíneas y/o cultivos de invierno), es una de las herramientas más importantes y válidas para potenciar el funcionamiento de los agroecosistemas. El propósito de este trabajo fue identificar la secuencia agrícola que contribuya a incrementar la actividad microbiana del suelo, en sintonía con las variables químicas edáficas. El estudio se llevó a cabo en un ensayo de larga duración iniciado en 1975 bajo siembra directa. De los tratamientos que conforman el ensayo sólo se seleccionaron cuatro secuencias de cultivos agrícolas: soja-soja (S-S), soja-trigo/soja (S-T/S), maíz-soja (M-S) y maíz-trigo/soja (M-T/S). Sobre las muestras de suelo se determinaron parámetros biológicos, como respiración microbiana y actividades enzimáticas, y parámetros químicos como contenido de materia orgánica y macronutrientes. El análisis conjunto de los parámetros dio como resultado una diferenciación entre las secuencias que incluyeron al cultivo invernal (S-T/S y M-T/S) de las secuencias basadas únicamente en cultivos de verano (S-S y M-S). De esta manera las funciones microbianas junto a la materia orgánica y los nutrientes del suelo, demostraron la importancia de la inclusión de trigo como cultivo de invierno en la rotación agrícola.</text>
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            <name>Date</name>
            <description>A point or period of time associated with an event in the lifecycle of the resource</description>
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                <text>2018</text>
              </elementText>
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            <name>Subject</name>
            <description>The topic of the resource</description>
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                <text>Parámetros microbianos y químicos, manejo agrícola, sustentabilidad</text>
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          <element elementId="48">
            <name>Source</name>
            <description>A related resource from which the described resource is derived</description>
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                <text>Ciencia del Suelo</text>
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            <name>Publisher</name>
            <description>An entity responsible for making the resource available</description>
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                <text>Asociación Argentina de la Ciencia del Suelo</text>
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            <name>Coverage</name>
            <description>The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant</description>
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                <text>Agriculture (General), Plant culture</text>
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            <description>A related resource</description>
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                <text>&lt;a href="http://www.scielo.org.ar/pdf/cds/v36n1/v36n1a08.pdf" target="_blank" rel="noreferrer noopener"&gt;http://www.scielo.org.ar/pdf/cds/v36n1/v36n1a08.pdf&lt;/a&gt;</text>
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                  <text>Agricultura sostenible</text>
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              <description>An account of the resource</description>
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                  <text>Dominio científico: Agricultura sostenible</text>
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      <name>Text</name>
      <description>A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.</description>
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            <name>Title</name>
            <description>A name given to the resource</description>
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                <text>Actitud hacia la gestión de residuos sólidos domiciliarios en estudiantes de una universidad privada</text>
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            <name>Creator</name>
            <description>An entity primarily responsible for making the resource</description>
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              <elementText elementTextId="229647">
                <text>Danny  Domínguez Del Águila, Mildred Teresa  Paredes Tarazona, Héctor Manuel  Hernández Valz</text>
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            <name>Description</name>
            <description>An account of the resource</description>
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                <text>Investigación descriptiva-comparativa con el objetivo de describir la actitud hacia la gestión de residuos sólidos domiciliarios en estudiantes de una universidad privada en la ciudad de Lima. Estudio en una muestra al azar, los colaboradores participaron previo consentimiento informado sobre el objetivo del estudio enmarcado en el índice percibido ambiental PEQI focalizado en la ac titud hacia la gestión de residuos sólidos. La data se obtuvo mediante la escala de actitudes MAARS-2019 tipo Osgood, con un índice omega de 0.956 a 0.976 cuya validez y confiabilidad es significativa. Los resultados indican que el grado de actitud es de 4.605, señalando como una actitud que tiende a la dirección positiva en la población estudiada hacia la gestión de los residuos sólidos domiciliarios, orientando así al diagnóstico favorable a lograr conductas ambientales para reducir su impacto ambiental que se viene observando en la generación de gases de efecto invernadero asociado al cambio climático y a la salud pública.</text>
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            <name>Date</name>
            <description>A point or period of time associated with an event in the lifecycle of the resource</description>
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                <text>2021</text>
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            <name>Subject</name>
            <description>The topic of the resource</description>
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                <text>actitud hacia el compostaje, actitud hacia la gestión de residuos sólidos, actitud hacia la segregación, ctitud hacia la reutilización y reducción de residuos sólidos</text>
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                <text>10.15381/iigeo.v24i47.20647</text>
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                  <text>Agricultura sostenible</text>
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              <description>An account of the resource</description>
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                  <text>Dominio científico: Agricultura sostenible</text>
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                <text>Danny Alfaro Rojas, Ian Portuguez Brenes, Héctor  Perdomo Velázquez, Roberto Vargas Masís</text>
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                <text>Introducción: Una de las manifestaciones de contaminación o perturbación ambiental que provoca la urbanización es la contaminación sonora o ruido. El ruido de forma general se relaciona con la presencia en el ambiente de niveles sonoros no deseados. Estos provocan en el ser humano y en la vida silvestre molestias y estrés y otros efectos nocivos en la salud. Objetivo: Se analizó el ruido presente en áreas verdes periurbanas y urbanas aledañas a la microcuenca del río Bermúdez en Costa Rica. Métodos: Se utilizó equipo de grabación automatizado (Audiomoth), el cual fue instalado en seis sitios de muestreo a lo largo de la microcuenca, las grabadoras se programaron para grabar 1 minuto cada 10 minutos durante 24 horas, a lo largo de 6 días continuos de grabación y en dos ocasiones por sitio. Resultados: Los niveles del ruido promedio de la zona urbana fueron significativamente más altos que en la zona periurbana (V= 3383238, gl =5465, P</text>
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                <text>10.22458/urj.v12i2.2846</text>
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                <text>Cuadernos de investigación UNED</text>
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            <description>An entity responsible for making the resource available</description>
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              <elementText elementTextId="153679">
                <text>Universidad Estatal a Distancia (UNED)</text>
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                <text>General Works</text>
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                <text>&lt;a href="https://revistas.uned.ac.cr/index.php/cuadernos/article/view/2846" target="_blank" rel="noreferrer noopener"&gt;https://revistas.uned.ac.cr/index.php/cuadernos/article/view/2846&lt;/a&gt;</text>
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                  <text>Dominio científico: Coronavirus</text>
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                <text>Arterivirus Nsp1 modulates the accumulation of minus-strand templates to control the relative abundance of viral mRNAs.</text>
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                <text>Danny D. Nedialkova, Alexander E Gorbalenya, Eric J. Snijder</text>
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                <text>The gene expression of plus-strand RNA viruses with a polycistronic genome depends on translation and replication of the genomic mRNA, as well as synthesis of subgenomic (sg) mRNAs. Arteriviruses and coronaviruses, distantly related members of the nidovirus order, employ a unique mechanism of discontinuous minus-strand RNA synthesis to generate subgenome-length templates for the synthesis of a nested set of sg mRNAs. Non-structural protein 1 (nsp1) of the arterivirus equine arteritis virus (EAV), a multifunctional regulator of viral RNA synthesis and virion biogenesis, was previously implicated in controlling the balance between genome replication and sg mRNA synthesis. Here, we employed reverse and forward genetics to gain insight into the multiple regulatory roles of nsp1. Our analysis revealed that the relative abundance of viral mRNAs is tightly controlled by an intricate network of interactions involving all nsp1 subdomains. Distinct nsp1 mutations affected the quantitative balance among viral mRNA species, and our data implicate nsp1 in controlling the accumulation of full-length and subgenome-length minus-strand templates for viral mRNA synthesis. The moderate differential changes in viral mRNA abundance of nsp1 mutants resulted in similarly altered viral protein levels, but progeny virus yields were greatly reduced. Pseudorevertant analysis provided compelling genetic evidence that balanced EAV mRNA accumulation is critical for efficient virus production. This first report on protein-mediated, mRNA-specific control of nidovirus RNA synthesis reveals the existence of an integral control mechanism to fine-tune replication, sg mRNA synthesis, and virus production, and establishes a major role for nsp1 in coordinating the arterivirus replicative cycle.</text>
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                <text>2010</text>
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                <text>DOI: 10.1371/journal.ppat.1000772</text>
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                <text>PLoS Pathogens</text>
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                <text>Biology (General), Immunologic diseases. Allergy</text>
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                  <text>Agricultura sostenible</text>
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              <description>An account of the resource</description>
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                  <text>Dominio científico: Agricultura sostenible</text>
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                <text>Sacarificación y fermentación simultánea de la cáscara de Jatropha curcas pretratada hidrotérmicamente  para producción de bioetanol</text>
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                <text>Danny Fabricio Sinche Arias, Valeria Estefanía Ramírez Peñaherrera, Cristhian Mauricio Velalcázar Rhea</text>
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            <description>An account of the resource</description>
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                <text>La biomasa proveniente de Jatropha curcas ha sido estudiada como materia prima para la obtención de energía renovable debido a que es un cultivo no alimentario. Uno de los procesos más prometedores para producir etanol a partir de biomasa lignocelulósica es la sacarificación y fermentación simultáneas (SSF). En otros estudios realizados, con experimentos SSF, se ha evaluado la conversión de glucano en etanol a partir de la cáscara de Jatropha pretratada en medio ácido o alcalino pero no a través de procesos hidrotérmicos. En el presente estudio, la cáscara de Jatropha curcas fue pretratada hidrotérmicamente con agua caliente a 200 °C por 15 min, y ensayada en experimentos de SSF con un coctel enzimático (Cellic CTec 2) y levadura comercial (Saccharomyces cerevisiae). Diferentes cargas enzimáticas, medios estériles y tamaños de ensayo fueron usados para determinar su influencia en el rendimiento de etanol. Los resultados mostraron que, con 5% p/p de sólidos, 15 UPF/g biomasa seca, y 1mg/ml de levadura se alcanza el máximo rendimiento de etanol (0,160- 0,177 g etanol/ g biomasa seca) y conversión de celulosa (57,71- 63,90%). A través de esta investigación se propone una alternativa para el uso integral del cultivo de Jatropha curcas dentro de una economía circular.</text>
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                <text>10.15517/ri.v32iNE1.46884</text>
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                <text>Universidad Distrital Francisco José de Caldas</text>
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                <text>Engineering (General). Civil engineering (General)</text>
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                <text>&lt;a href="https://revistas.ucr.ac.cr/index.php/ingenieria/article/view/46884" target="_blank" rel="noreferrer noopener"&gt;https://revistas.ucr.ac.cr/index.php/ingenieria/article/view/46884&lt;/a&gt;</text>
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                <text>COVID-19: what the clinician should know about post-mortem findings.</text>
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                <text>Intensive care medicine</text>
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                  <text>Dominio científico: Coronavirus</text>
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                <text>Commentary: Coronavirus disease 2019 in cardiac surgery: We are still learning and have yet to see all possible complications.</text>
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                <text>Danny Ramzy</text>
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                <text>2020</text>
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                <text>10.1016/j.xjtc.2020.10.007</text>
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                <text>JTCVS techniques</text>
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              <name>Title</name>
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                  <text>Agricultura sostenible</text>
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              <name>Description</name>
              <description>An account of the resource</description>
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                  <text>Dominio científico: Agricultura sostenible</text>
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      <name>Text</name>
      <description>A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.</description>
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        <name>Dublin Core</name>
        <description>The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.</description>
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            <name>Title</name>
            <description>A name given to the resource</description>
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                <text>Rango altitudinal: factor de vigor forestal y determinante en la regeneración natural del oyamel</text>
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            <name>Creator</name>
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                <text>Dante Arturo Rodríguez-Trejo, Alejandro Ismael Monterroso Rivas, Luis Felipe Romahn-Hernández, Antonio Villanueva-Morales, María de Jesús Pérez-Hernández</text>
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          <element elementId="41">
            <name>Description</name>
            <description>An account of the resource</description>
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                <text>Objetivo: evaluar el vigor del arbolado y la regeneración natural de oyamel al oriente del Estado de México, considerando variables topográficas y forestales. Método: se usó muestreo sistemático, con sitios cada 100 m s. n. m. entre los 2900 y 3600 m s. n. m. Con el programa sas se hicieron pruebas de normalidad, tablas de frecuencia y análisis de varianza para analizar la relación entre altitud, vigor y regeneración de Abies religiosa (Kunth) Schltdl. et Cham. Resultados: hay relación entre la altitud, el vigor y la densidad arbórea. La regeneración es nula en las cotas más bajas e incrementa con la altitud hasta los 3 400 m s. n. m. y no muestra gran reducción más arriba. La densidad de los estratos A (dap ≥12.5 cm) y B (dap 1 m de altura) incrementa conforme a la altitud; las densidades más altas se registran en la cota 3 400 m s. n. m. Limitaciones: se requiere investigación de otros factores que intervienen en la distribución de la especie. Principales hallazgos: la regeneración tiene más éxito en zonas medias y altas, y la condición del arbolado tiende a ser mejor ahí también. Se evidencia que el oyamel migra a zonas más elevadas, debido al cambio climático global.</text>
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            <name>Date</name>
            <description>A point or period of time associated with an event in the lifecycle of the resource</description>
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                <text>2020</text>
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            <name>Subject</name>
            <description>The topic of the resource</description>
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                <text>Abies religiosa, Regeneración natural, calentamiento global, declinación forestal, rango altitudinal</text>
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          <element elementId="43">
            <name>Identifier</name>
            <description>An unambiguous reference to the resource within a given context</description>
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              <elementText elementTextId="199060">
                <text>10.22201/enesl.20078064e.2020.22.72751e22.72751</text>
              </elementText>
            </elementTextContainer>
          </element>
          <element elementId="48">
            <name>Source</name>
            <description>A related resource from which the described resource is derived</description>
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              <elementText elementTextId="199061">
                <text>Entreciencias: Diálogos en la Sociedad del Conocimiento</text>
              </elementText>
            </elementTextContainer>
          </element>
          <element elementId="45">
            <name>Publisher</name>
            <description>An entity responsible for making the resource available</description>
            <elementTextContainer>
              <elementText elementTextId="199062">
                <text>Universidad Nacional Autónoma de México</text>
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          </element>
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            <name>Coverage</name>
            <description>The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant</description>
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              <elementText elementTextId="199063">
                <text>Social Sciences, Science</text>
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            <name>Relation</name>
            <description>A related resource</description>
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                <text>&lt;a href="http://www.redalyc.org/articulo.oa?id=457662386014" target="_blank" rel="noreferrer noopener"&gt;http://www.redalyc.org/articulo.oa?id=457662386014&lt;/a&gt;</text>
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