http://socictopen.socict.org/files/original/985a36c4caa0053237442890469f75a8.pdf f33440724da2c07fa2c448bf578abccb Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Agricultura sostenible Description An account of the resource Dominio científico: Agricultura sostenible Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Cytotoxicity of Agaricus sylvaticus in non-tumor cells (NTH/3T3) and tumor (OSCC-3) using tetrazolium (MTT) assay Creator An entity primarily responsible for making the resource Joice Vinhal Costa Orsine, Luíssa Marques Brito, Renata Carvalho Silva, Maria de Fátima Menezes Santos Almeida, Maria Rita Carvalho Garbi Novaes Description An account of the resource The purpose of this study was to assess the cytotoxic effect of the non-fractionated aqueous extract of A. sylvaticus mushroom in cultures of non-tumor cells (NIH3T3) and tumor cells (OSCC-3). The cells were maintained in DMEN cell culture medium added of 10% of fetal bovine serum and 1% antibiotic. For the cytotoxicity test we prepared the aqueous mushroom extract at concentrations of 0.01 mg.ml-1, 0.02 mg.ml-1, 0.04 mg.ml-1, 0.08 mg.ml-1, 0.16 mg.ml-1, and 0.32 mg.ml-1. For the culture, 2 x 10(5) cells/ml was deposited in 96-well microplates during 24 hour incubation with subsequent exchange of medium by another containing the mushroom concentrations. After 24 hour incubation the medium was discarded and 100 ml of tetrazolium blue (MTT) was added at a concentration of 5 mg.ml-1. The microplates were incubated for 2 h at 37º C. Spectrophotometric analysis was performed using 570 nm wavelength. From the values of the optical densities we determined the drug concentration capable of reducing cell viability by 50%. Therefore, the mushroom A. sylvaticus, at all concentrations tested, did not show cytotoxic effects, once the inhibitory concentration (IC50) obtained for tumor cells OSCC-3 was 0.06194 mg.ml-1, and the IC50 checked for non-tumor cells NIH3T3 was 0,06468 mg.ml-1. This test made it possible to determine that A. sylvaticus mushroom has no cytotoxic effects, suggesting its use safe for human consumption. Date A point or period of time associated with an event in the lifecycle of the resource 2013 Subject The topic of the resource Agaricus sylvaticus, seguridad alimentaria, toxicidad Identifier An unambiguous reference to the resource within a given context 10.3305/nh.2013.28.4.6461 Source A related resource from which the described resource is derived Nutrición Hospitalaria Publisher An entity responsible for making the resource available Arán Ediciones, S. L. Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Nutritional diseases. Deficiency diseases Relation A related resource <a href="http://scielo.isciii.es/scielo.php?script=sci_arttext&amp;pid=S0212-16112013000400038&amp;lng=en&amp;tlng=en" target="_blank" rel="noreferrer noopener">http://scielo.isciii.es/scielo.php?script=sci_arttext&amp;pid=S0212-16112013000400038&amp;lng=en&amp;tlng=en</a>