http://socictopen.socict.org/files/original/9e9737b47ea386187f8d0a4f1389664e.pdf 74c6422c06e21ba06492c2c61cceefb7 Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Agricultura sostenible Description An account of the resource Dominio científico: Agricultura sostenible Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Lack of correlation between micro fungi species and chemical control method of Atta treated with toxic baits Creator An entity primarily responsible for making the resource Marcílio Souza Barbosa, Suzanne Nunes Barbosa, Nilson Satoru Nagamoto, Luiz Carlos Forti, Cícero lmeida Description An account of the resource ABSTRACT: Atta sexdens rubropilosa (leaf-cutter ants) has a symbiotic association with a fungus and has a negative interaction with other fungi due to parasitism of the fungus cultivated by ants; also, there are several other fungi with no exact known role occurring in their cultivated fungus garden. In the present study, we use the ITS region (internal transcribed spacer) to identify fungi in colonies treated with toxic baits. Experiments using two toxic baits were carried out: 0.75g of sulfluramid [0.3%] and 0.75g fipronil [0.003%]. Samples of fungi were collected and cultured in Czapek medium for seven days to allow fungal growth and subsequent identification. Total DNA was isolated from 100-150 mg of mycelium using the CTAB method and using PCR, with the universal primers (ITS4 and ITS5), to amplify the ITS region. Sequencing was performed using the Sanger method. Sequences were subjected to BLAST, allowing the identification of nine different species of the orders Agaricales, Eurotiales, Hypocreales, Pleosporales, Saccharomycetales and Tremellales showing a variation in identity of 96-100%. Using “The Automatic Barcode Gap Discovery” analysis, nine groups were identified, corresponding to species described in NCBI. The K2P distances were used to generate a tree using Neighbour-joining, demonstrating that the species were grouped according to phylogenetic groups. We concluded that leaf-cutter ant colonies exhibited a wide variety of fungi and this study suggested that there is no correlation between the species of fungi isolated with the control method used on the ant nest. Date A point or period of time associated with an event in the lifecycle of the resource 2018 Subject The topic of the resource Atta, DNA barcoding, ITS, ecología, formigas cortadeira Identifier An unambiguous reference to the resource within a given context 10.1590/0103-8478cr20170353 Source A related resource from which the described resource is derived Ciência Rural Publisher An entity responsible for making the resource available Universidade Federal de Santa Maria Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Agriculture (General), Agriculture Relation A related resource <a href="http://www.scielo.br/scielo.php?script=sci_arttext&amp;pid=S0103-84782018000500150&amp;lng=en&amp;tlng=en" target="_blank" rel="noreferrer noopener">http://www.scielo.br/scielo.php?script=sci_arttext&amp;pid=S0103-84782018000500150&amp;lng=en&amp;tlng=en</a>