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                <text>Dominio científico: Coronavirus</text>
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              <text>A Real-Time PCR Assay for Bat SARS-Like Coronavirus Detection and Its Application to Italian Greater Horseshoe Bat Faecal Sample Surveys</text>
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              <text>Laura Gallina, Santino Prosperi, Andrea Balboni, Mara Battilani, Alessandra Palladini</text>
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              <text>Bats are source of coronaviruses closely related to the severe acute respiratory syndrome (SARS) virus. Numerous studies have been carried out to identify new bat viruses related to SARS-coronavirus (bat-SARS-like CoVs) using a reverse-transcribed-polymerase chain reaction assay. However, a qualitative PCR could underestimate the prevalence of infection, affecting the epidemiological evaluation of bats in viral ecology. In this work an SYBR Green-real time PCR assay was developed for diagnosing infection with SARS-related coronaviruses from bat guano and was applied as screening tool in a survey carried out on 45 greater horseshoe bats (Rhinolophus ferrumequinum) sampled in Italy in 2009. The assay showed high sensitivity and reproducibility. Its application on bats screening resulted in a prevalence of 42%. This method could be suitable as screening tool in epidemiological surveys about the presence of bat-SARS-like CoVs, consequently to obtain a more realistic scenario of the viral prevalence in the population.</text>
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              <text>2012</text>
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              <text>DOI: 10.1100/2012/989514</text>
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              <text>The Scientific World Journal</text>
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              <text>Hindawi Limited</text>
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          <name>Coverage</name>
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              <text>Technology, Science, Medicine</text>
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