Comparative Activity of Six Recombinant Stilbene Synthases in Yeast for Resveratrol Production
subject: 4CL, STS, engineered yeasts, resveratrol production
description: Resveratrol is a nutraceutical with relevant benefits to human health. This investigation reports on the generation and evaluation of six recombinant yeast lines that produce resveratrol from p-coumaric acid. The yeast lines contained a single p-coumaric acid-Co-A ligase from Plagiochasma appendiculatum combined with the stilbene synthases from Parthenocissus henryana, Polygonum cuspidatum, Morus alba var. atropurpurea, Rheum tataricum, Vitis vinifera and Arachis hypogaea. Codon optimized versions of these sequences were inserted in an expression vector flanked by the constitutive PGK and GPD promoters before expression in Saccharomyces cerevisiae. Batch fermentation (60 h) revealed that yeast lines had different capacities (p < 0.01) to produce resveratrol. Slightly acidic pH (6) and concentrations
creator: Nemesio Villa-Ruano, Antonio Rivera, Efraín Rubio-Rosas, Gerardo Landeta-Cortés, Jenaro Leocadio Varela-Caselis, Omar Romero-Arenas
source: Applied Sciences
publisher: MDPI AG
date: 2020
relation: https://www.mdpi.com/2076-3417/10/14/4847
coverage: Engineering (General). Civil engineering (General), Technology, Biology (General), Chemistry, Physics