Triplex Real-Time RT-PCR for Severe Acute Respiratory Syndrome Coronavirus 2.
subject: Respiratory Infections, Viruses, Zoonoses, coronavirus, molecular detection, SARS, rRT-PCR, SARS-CoV-2, COVID-19, Severe Acute Respiratory Syndrome Coronavirus 2, 2019 novel coronavirus disease, Coronavirus disease
description: Most reverse transcription PCR protocols for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) include 2-3 targets for detection. We developed a triplex, real-time reverse transcription PCR for SARS-CoV-2 that maintained clinical performance compared with singleplex assays. This protocol could streamline detection and decrease reagent use during current high SARS-CoV-2 testing demands.
creator: Jesse J. Waggoner, Malaya K. Sahoo, Benjamin A. Pinsky, Ahmed Babiker, Evan J. Anderson, Nadine Rouphael, Colleen S. Kraft, Victoria Stittleburg, Renee Pond, Youssef Saklawi, Laila Hussaini
source: Emerging Infectious Diseases
publisher: Centers for Disease Control and Prevention
date: 2020
identifier: DOI: 10.3201/eid2607.201285