Optimization of the CDC Protocol of Molecular Diagnosis of COVID-19 for Timely Diagnosis.
subject: molecular diagnostics, Coronavirus disease 2019 (COVID-19), real-time reverse transcriptase polymerase chain (RT-PCR), severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
description: Coronavirus disease 2019 (COVID-19), the current uncontrolled outbreak of infectious disease, has caused significant challenges throughout the world. A reliable rapid diagnostic test for COVID-19 is demanded worldwide. The real-time reverse transcriptase polymerase chain was one of the most quickly established methods in the novel viral pandemic and was considered as the gold standard for the detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). In this report, we illustrate our experience of applying a protocol from the Taiwan CDC and achieving assay optimization in the immediate circumstances to meet the urgent medical and public health needs.
creator: Yihui Huang, Po-Liang Lu, Chu-Feng Wang, Shang-Yi Lin, Shu-kai Lin, Chao-Ju Chen, Li-Ling Hsieh
source: Diagnostics
publisher: MDPI AG
date: 2020
identifier: DOI: 10.3390/diagnostics10050333