Real-Time Polymerase Chain Reaction for Detecting SARS Coronavirus, Beijing, 2003
subject: SARS-CoV, PCR, Diagnostic, China
description: During the 2003 severe acute respiratory syndrome (SARS) outbreak, a real-time quantitative polymerase chain reaction, which targets the nucleocapsid gene at the 3′-end of the viral genome, was established to detect and identify the SARS-associated coronavirus. We describe the use of this assay to screen >700 clinical samples.
creator: Junhui Zhai, Thomas Briese, Erhei Dai, Xiaoyi Wang, Xin Pang, Zongmin Du, Haihong Liu, Jin Wang, Hongxia Wang, Zhaobiao Guo, Zeliang Chen, Lingxiao Jiang, Dongsheng Zhou, Yanping Han, Omar Jabado, Gustavo Palacios, W. Ian Lipkin, Ruifu Yang
source: Emerging Infectious Diseases
publisher: Centers for Disease Control and Prevention
date: 2004
language: EN
identifier: DOI: 10.3201/eid1002.030799
coverage: Infectious and parasitic diseases, Medicine