57 10 20655 https://socictopen.socict.org/files/original/49f6db2e249bd47b4c713439ef48f721.pdf f58bf20cf1196dd808a9cc109be40ec0 Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Coronavirus Description An account of the resource Dominio científico: Coronavirus Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource A mouse-adapted SARS-coronavirus causes disease and mortality in BALB/c mice. Creator An entity primarily responsible for making the resource Anjeanette Roberts, Damon Deming, Christopher D. Paddock, Aaron Cheng, Boyd Yount, Leatrice Vogel, Brian D Herman, Tim Sheahan, Mark Heise, Gillian L. Genrich, Sherif R. Zaki, Ralph Baric, Kanta Subbarao Description An account of the resource No single animal model for severe acute respiratory syndrome (SARS) reproduces all aspects of the human disease. Young inbred mice support SARS-coronavirus (SARS-CoV) replication in the respiratory tract and are available in sufficient numbers for statistical evaluation. They are relatively inexpensive and easily accessible, but their use in SARS research is limited because they do not develop illness following infection. Older (12- to 14-mo-old) BALB/c mice develop clinical illness and pneumonitis, but they can be hard to procure, and immune senescence complicates pathogenesis studies. We adapted the SARS-CoV (Urbani strain) by serial passage in the respiratory tract of young BALB/c mice. Fifteen passages resulted in a virus (MA15) that is lethal for mice following intranasal inoculation. Lethality is preceded by rapid and high titer viral replication in lungs, viremia, and dissemination of virus to extrapulmonary sites accompanied by lymphopenia, neutrophilia, and pathological changes in the lungs. Abundant viral antigen is extensively distributed in bronchial epithelial cells and alveolar pneumocytes, and necrotic cellular debris is present in airways and alveoli, with only mild and focal pneumonitis. These observations suggest that mice infected with MA15 die from an overwhelming viral infection with extensive, virally mediated destruction of pneumocytes and ciliated epithelial cells. The MA15 virus has six coding mutations associated with adaptation and increased virulence; when introduced into a recombinant SARS-CoV, these mutations result in a highly virulent and lethal virus (rMA15), duplicating the phenotype of the biologically derived MA15 virus. Intranasal inoculation with MA15 reproduces many aspects of disease seen in severe human cases of SARS. The availability of the MA15 virus will enhance the use of the mouse model for SARS because infection with MA15 causes morbidity, mortality, and pulmonary pathology. This virus will be of value as a stringent challenge in evaluation of the efficacy of vaccines and antivirals. Date A point or period of time associated with an event in the lifecycle of the resource 2007 Identifier An unambiguous reference to the resource within a given context DOI: 10.1371/journal.ppat.0030005 Source A related resource from which the described resource is derived PLoS Pathogens Publisher An entity responsible for making the resource available Public Library of Science (PLoS) Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Biology (General), Immunologic diseases. Allergy Language A language of the resource EN https://socictopen.socict.org/files/original/963225489d818e72bdd34bdcb2262e5d.pdf 1be93a3208edc3d4bb24521ac15abe46 Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Agricultura sostenible Description An account of the resource Dominio científico: Agricultura sostenible Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource A Mudança da Monocultura do Fumo no Agreste Alagoano: Seus Arranjos Produtivos Locais e os Impactos na Estrutura Social Creator An entity primarily responsible for making the resource José Senivaldo Liberato Description An account of the resource Durante décadas a cultura do tabaco proporcionou a geração de emprego e renda, sobretudo a formação de riqueza para poucas famílias detentoras dos meios de beneficiamento e produção, na região do agreste alagoano. A agricultura familiar utilizava de suas terras para o plantio de uma única cultura agrícola, sem a possibilidade de arbitrar sobre os valores de compra e venda, uma vez que sua colheita era, geralmente, com destino a um único comprador. Com a questão de saúde pública sobre a redução do consumo de tabaco, as quedas nas vendas internas e externas, fizeram com que o governo brasileiro desenvolvesse políticas públicas voltadas para o desenvolvimento social e econômico com ênfase na diversificação da agricultura familiar, surgindo assim os arranjos produtivos locais e uma nova estrutura social. Date A point or period of time associated with an event in the lifecycle of the resource 2016 Subject The topic of the resource Arranjo produtivo local, Desenvolvimento sustentável, Estrutura Social, Sociologia econômica Source A related resource from which the described resource is derived International Journal of Professional Business Review Publisher An entity responsible for making the resource available Tutor Treinamento e Desenvolvimento Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Business, Management. Industrial management Relation A related resource <a href="http://openaccessojs.com/index.php/JBReview/article/view/17" target="_blank" rel="noreferrer noopener">http://openaccessojs.com/index.php/JBReview/article/view/17</a> https://socictopen.socict.org/files/original/f5915b77d48e55f9f71cede7a042e117.pdf 662ca99e67cac10f4f41b3a96627f1ed Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Agricultura sostenible Description An account of the resource Dominio científico: Agricultura sostenible Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource A mulher na agricultura orgânica e em novas ruralidades The role of the woman farmer in the emergency of new ruralities: notes from the organic farming Creator An entity primarily responsible for making the resource Karen Follador Karam Description An account of the resource Conhecer e reconhecer o papel da mulher agricultora nos novos processos produtivos em curso voltados a atualizar o lugar do rural nas sociedades contemporâneas, a partir do sistema de produção da agricultura orgânica, é a pretensão maior deste trabalho. Interessa identificar as estratégias adotadas pela agricultura familiar para a dinamização e manutenção de modos de viver o meio rural, ao mesmo tempo que interessa identificar as possibilidades de trazer à visibilidade o espaço rural nas sociedades modernas. Nesse contexto é que se pesquisou a agricultura orgânica na Região Metropolitana de Curitiba (RMC), analisou-se quem são os agricultores envolvidos no processo e, dentro dele, buscou-se apontar o papel que a mulher agricultora desempenha, desde o processo produtivo até a sociabilidade necessária à manutenção de um 'meio rural vivo', onde a agricultura orgânica aparece como uma estratégia.The main pretension in this work is to get to know and to recognize the role of the woman farmer in the new productive processes that update the place of the rural in the contemporary societies. The starting point is the organic farming system. One interests to identify the strategies adopted by the family farming for increasing and supporting ways of living the rural world. At the same time, one interests to identify the possibilities to make the rural space - in the context of the modern societies - visible. In this context, one researched the organic farming in the urban area of Curitiba (RMC). One analyzed who are the farmers involved in the process, and inside of it, one tried to indicate what is the role of the woman farmer, from the productive process to the necessary sociability to keep an 'alive rural world' where the organic farming appears as a strategy. Date A point or period of time associated with an event in the lifecycle of the resource 2004 Subject The topic of the resource Agricultura orgânica, Ruralidade, mulher agricultora, organic farming, rurality, woman farmer Identifier An unambiguous reference to the resource within a given context 10.1590/S0104-026X2004000100016 Source A related resource from which the described resource is derived Revista Estudos Feministas Publisher An entity responsible for making the resource available Universidade Federal de Santa Catarina Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Women. Feminism Relation A related resource <a href="http://www.scielo.br/scielo.php?script=sci_arttext&amp;pid=S0104-026X2004000100016" target="_blank" rel="noreferrer noopener">http://www.scielo.br/scielo.php?script=sci_arttext&amp;pid=S0104-026X2004000100016</a> https://socictopen.socict.org/files/original/8a97ba26141eee4ef79237bd9a94006f.pdf e2c8d03dabecbf08725a6811239a1b84 Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Coronavirus Description An account of the resource Dominio científico: Coronavirus Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource A multi-method approach to curriculum development for in-service training in China's newly established health emergency response offices. Creator An entity primarily responsible for making the resource Yadong Wang, Xiangrui Li, Yiwen Yuan, Mahomed S Patel Description An account of the resource To describe an innovative approach for developing and implementing an in-service curriculum in China for staff of the newly established health emergency response offices (HEROs), and that is generalisable to other settings.The multi-method training needs assessment included reviews of the competency domains needed to implement the International Health Regulations (2005) as well as China's policies and emergency regulations. The review, iterative interviews and workshops with experts in government, academia, the military, and with HERO staff were reviewed critically by an expert technical advisory panel.Over 1600 participants contributed to curriculum development. Of the 18 competency domains identified as essential for HERO staff, nine were developed into priority in-service training modules to be conducted over 2.5 weeks. Experts from academia and experienced practitioners prepared and delivered each module through lectures followed by interactive problem-solving exercises and desktop simulations to help trainees apply, experiment with, and consolidate newly acquired knowledge and skills.This study adds to the emerging literature on China's enduring efforts to strengthen its emergency response capabilities since the outbreak of SARS in 2003. The multi-method approach to curriculum development in partnership with senior policy-makers, researchers, and experienced practitioners can be applied in other settings to ensure training is responsive and customized to local needs, resources and priorities. Ongoing curriculum development should reflect international standards and be coupled with the development of appropriate performance support systems at the workplace for motivating staff to apply their newly acquired knowledge and skills effectively and creatively. Date A point or period of time associated with an event in the lifecycle of the resource 2014 Identifier An unambiguous reference to the resource within a given context DOI: 10.1371/journal.pone.0100892 Source A related resource from which the described resource is derived PLoS ONE Publisher An entity responsible for making the resource available Public Library of Science (PLoS) Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Science, Medicine Language A language of the resource EN https://socictopen.socict.org/files/original/1dc052b22779c2a81c6b730876dda135.pdf e92cad4f16aba4cc4edb4ee0763245a4 Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Agricultura sostenible Description An account of the resource Dominio científico: Agricultura sostenible Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource A Multi-Omics Analysis Pipeline for the Metabolic Pathway Reconstruction in the Orphan Species Quercus ilex Creator An entity primarily responsible for making the resource Cristina López-Hidalgo, Victor M. Guerrero-Sánchez, Isabel Gómez-Gálvez, Rosa Sánchez-Lucas, María A. Castillejo-Sánchez, Ana M. Maldonado-Alconada, Luis Valledor, Jesus V. Jorrín-Novo Description An account of the resource Holm oak (Quercus ilex) is the most important and representative species of the Mediterranean forest and of the Spanish agrosilvo-pastoral “dehesa” ecosystem. Despite its environmental and economic interest, Holm oak is an orphan species whose biology is very little known, especially at the molecular level. In order to increase the knowledge on the chemical composition and metabolism of this tree species, the employment of a holistic and multi-omics approach, in the Systems Biology direction would be necessary. However, for orphan and recalcitrant plant species, specific analytical and bioinformatics tools have to be developed in order to obtain adequate quality and data-density before to coping with the study of its biology. By using a plant sample consisting of a pool generated by mixing equal amounts of homogenized tissue from acorn embryo, leaves, and roots, protocols for transcriptome (NGS-Illumina), proteome (shotgun LC-MS/MS), and metabolome (GC-MS) studies have been optimized. These analyses resulted in the identification of around 62629 transcripts, 2380 protein species, and 62 metabolites. Data are compared with those reported for model plant species, whose genome has been sequenced and is well annotated, including Arabidopsis, japonica rice, poplar, and eucalyptus. RNA and protein sequencing favored each other, increasing the number and confidence of the proteins identified and correcting erroneous RNA sequences. The integration of the large amount of data reported using bioinformatics tools allows the Holm oak metabolic network to be partially reconstructed: from the 127 metabolic pathways reported in KEGG pathway database, 123 metabolic pathways can be visualized when using the described methodology. They included: carbohydrate and energy metabolism, amino acid metabolism, lipid metabolism, nucleotide metabolism, and biosynthesis of secondary metabolites. The TCA cycle was the pathway most represented with 5 out of 10 metabolites, 6 out of 8 protein enzymes, and 8 out of 8 enzyme transcripts. On the other hand, gaps, missed pathways, included metabolism of terpenoids and polyketides and lipid metabolism. The multi-omics resource generated in this work will set the basis for ongoing and future studies, bringing the Holm oak closer to model species, to obtain a better understanding of the molecular mechanisms underlying phenotypes of interest (productive, tolerant to environmental cues, nutraceutical value) and to select elite genotypes to be used in restoration and reforestation programs, especially in a future climate change scenario. Date A point or period of time associated with an event in the lifecycle of the resource 2018 Subject The topic of the resource Quercus ilex, metabolome, omics, proteome, transcriptome Identifier An unambiguous reference to the resource within a given context 10.3389/fpls.2018.00935 Source A related resource from which the described resource is derived Frontiers in Plant Science Publisher An entity responsible for making the resource available Frontiers Media S.A. Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Plant culture Relation A related resource <a href="https://www.frontiersin.org/article/10.3389/fpls.2018.00935/full" target="_blank" rel="noreferrer noopener">https://www.frontiersin.org/article/10.3389/fpls.2018.00935/full</a> https://socictopen.socict.org/files/original/538e40657b1d835ba7b34b323cb460c0.pdf 068ec5e1621c2f1bb4d3d37febb29c39 Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Coronavirus Description An account of the resource Dominio científico: Coronavirus Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource A multi-targeting drug design strategy for identifying potent anti-SARS-CoV-2 inhibitors. Creator An entity primarily responsible for making the resource Weijuan Shang, Leike Zhang, Peng-Xuan Ren, Wan-Chao Yin, Huan Ge, Lin Wang, Xiang-Lei Zhang, Bing-Qian Li, Hong-Lin Li, Ye-Chun Xu, Eric H Xu, Hua-Liang Jiang, Li-Li Zhu, Fang Bai Description An account of the resource The COVID-19, caused by SARS-CoV-2, is threatening public health, and there is no effective treatment. In this study, we have implemented a multi-targeted anti-viral drug design strategy to discover highly potent SARS-CoV-2 inhibitors, which simultaneously act on the host ribosome, viral RNA as well as RNA-dependent RNA polymerases, and nucleocapsid protein of the virus, to impair viral translation, frameshifting, replication, and assembly. Driven by this strategy, three alkaloids, including lycorine, emetine, and cephaeline, were discovered to inhibit SARS-CoV-2 with EC50 values of low nanomolar levels potently. The findings in this work demonstrate the feasibility of this multi-targeting drug design strategy and provide a rationale for designing more potent anti-virus drugs. Date A point or period of time associated with an event in the lifecycle of the resource 2021 Subject The topic of the resource RdRp, SARS-CoV-2 inhibitors, Virus RNA, host ribosome Identifier An unambiguous reference to the resource within a given context 10.1038/s41401-021-00668-7 Source A related resource from which the described resource is derived Acta pharmacologica Sinica https://socictopen.socict.org/files/original/d9378580b594e7b00070f6110526b72b.pdf 95f40ea0c690d17a7afc11349c98678b Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Coronavirus Description An account of the resource Dominio científico: Coronavirus Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource A multicentre observational study on neonates exposed to SARS-CoV-2 in China: the Neo-SARS-CoV-2 Study protocol Creator An entity primarily responsible for making the resource Xiuyong Cheng, Wenhao Zhou, Wei Zhou, Xiao Chen, Long Li, Laishuan Wang, Tiantian Xiao, Shiwen Xia, Linkong Zeng, Guang Lin, Qiufen Wei, Deyi Zhuang, Bin Yi, Hongying Mi, Zhaoqing Yin, Xiaojing Hu Description An account of the resource Introduction An outbreak of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) occurred in Wuhan, China starting in December 2019. Yet the clinical features and long-term outcomes of neonates with SARS-CoV-2 exposure are lacking. The purpose of this study is to describe the clinical course and prognosis of the neonates exposed to SARS-CoV-2.Methods and analysis This is a multicentre observational study conducted at the designated children and maternal and child hospitals in the mainland of China. Neonates exposed to SARS-CoV-2 infection will be recruited. The data to be collected via case report forms include demographic details, clinical features, laboratory and imaging results, as well as outcomes. Primary outcomes are the mortality of neonates with COVID-19 and SARS-CoV-2 infection of neonates born to mothers with COVID-19. Secondary outcomes are the birth weight, premature delivery and neurological development of neonates exposed to SARS-CoV-2. The neurological development is assessed by the Chinese standardised Denver Developmental Screening Test at the corrected age of 6 months.Ethics and dissemination This study has been approved by the Children's Hospital of Fudan University ethics committee (No. (2020)31). The study findings will be disseminated in peer-reviewed journals and presented at national and international conferences in order to improve the understanding of the clinical course among neonates exposed to SARS-CoV-2 and to provide evidence-based treatment and prevention strategies for this group.Trial registration number NCT04279899. Date A point or period of time associated with an event in the lifecycle of the resource 2020 Identifier An unambiguous reference to the resource within a given context 10.1136/bmjopen-2020-038004 Source A related resource from which the described resource is derived Epidemiology and Health Publisher An entity responsible for making the resource available Korean Society of Epidemiology Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Medicine https://socictopen.socict.org/files/original/03305b83c9318bbf5c3901051d535ba4.pdf 5ae0597d45522dcc594fbd3a37155da3 Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Coronavirus Description An account of the resource Dominio científico: Coronavirus Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource A Multidimensional Model of Public Health Approaches Against COVID-19 Creator An entity primarily responsible for making the resource Iftikhar Hussain, Jian Tian, Sabahat Akram, Muhammad Afzal Shad, Mehrab Nazir, Sidney Mangenda Tshiaba, Shahrukh Mushtaq Description An account of the resource COVID-19 is appearing as one of the most fetal disease of the world’s history and has caused a global health emergency. Therefore, this study was designed with the aim to address the issue of public response against COVID-19. The literature lacks studies on social aspects of COVID-19. Therefore, the current study is an attempt to investigate its social aspects and suggest a theoretical structural equation model to examine the associations between social media exposure, awareness, and information exchange and preventive behavior and to determine the indirect as well as direct impact of social media exposure on preventive behavior from the viewpoints of awareness and information exchange. The current empirical investigation was held in Pakistan, and the collected survey data from 500 respondents through social media tools were utilized to examine the associations between studied variables as stated in the anticipated study model. The findings of the study indicate that social media exposure has no significant and direct effect on preventive behavior. Social media exposure influences preventive behavior indirectly through awareness and information exchange. In addition, awareness and information exchange have significant and direct effects on preventive behavior. Findings are valuable for health administrators, governments, policymakers, and social scientists, specifically for individuals whose situations are like those in Pakistan. This research validates how social media exposure indirectly effects preventive behavior concerning COVID-19 and explains the paths of effect through awareness or information exchange. To the best of our knowledge, there is no work at present that covers this gap, for this reason the authors propose a new model. The conceptual model offers valuable information for policymakers and practitioners to enhance preventive behavior through the adoption of appropriate awareness strategies and information exchange and social media strategies. Date A point or period of time associated with an event in the lifecycle of the resource 2020 Subject The topic of the resource social media, Pandemic, social actors, preventive behavior, COVID-19 Identifier An unambiguous reference to the resource within a given context DOI: 10.3390/ijerph17113780 Source A related resource from which the described resource is derived International Journal of Environmental Research and Public Health Publisher An entity responsible for making the resource available MDPI AG Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Medicine https://socictopen.socict.org/files/original/39edf97ed17b53d9b5f05af8fdc3a9d3.pdf 94ffb8e465e3bf9a861bad5a81df4514 Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Coronavirus Description An account of the resource Dominio científico: Coronavirus Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource A Multigene Approach for Comparing Genealogy of Betacoronavirus from Cattle and Horses Creator An entity primarily responsible for making the resource Paulo E. Brandão, Leonardo J. Richtzenhain, Karen M. Asano, Sibele P. Souza, Sheila O.S. Silva, Iracema N. Barros, Francisco S. Nogueira Neto Description An account of the resource Gastroenteritis is one of the leading causes of morbidity and mortality among young and newborn animals and is often caused by multiple intestinal infections, with rotavirus and bovine coronavirus (BCoV) being the main viral causes in cattle. Given that BCoV is better studied than equine coronaviruses and given the possibility of interspecies transmission of these viruses, this research was designed to compare the partial sequences of the spike glycoprotein (S), hemagglutinin-esterase protein (HE), and nucleoprotein (N) genes from coronaviruses from adult cattle with winter dysentery, calves with neonatal diarrhea, and horses. To achieve this, eleven fecal samples from dairy cows with winter dysentery, three from calves, and two from horses, all from Brazil, were analysed. It could be concluded that the enteric BCoV genealogy from newborn and adult cattle is directly associated with geographic distribution patterns, when S and HE genes are taken into account. A less-resolved genealogy exists for the HE and N genes in cattle, with a trend for an age-related segregation pattern. The coronavirus strains from horses revealed Betacoronavirus sequences indistinguishable from those found in cattle, a fact previously unknown. Date A point or period of time associated with an event in the lifecycle of the resource 2013 Identifier An unambiguous reference to the resource within a given context DOI: 10.1155/2013/349702 Source A related resource from which the described resource is derived The Scientific World Journal Publisher An entity responsible for making the resource available Hindawi Limited Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Technology, Science, Medicine https://socictopen.socict.org/files/original/41c01c11d54941bd6b7cb87770a513bc.pdf 546641362d7065b3d01a116f2a0427d5 Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Coronavirus Description An account of the resource Dominio científico: Coronavirus Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource A multiplex reverse transcription-nested polymerase chain reaction for detection and differentiation of wild-type and vaccine strains of canine distemper virus Creator An entity primarily responsible for making the resource Cui Shangjin, Wang Zhao, Zhou Shun, Si Wei Description An account of the resource Abstract A multiplex reverse transcription-nested polymerase chain reaction (RT-nPCR) method was developed for the detection and differentiation of wild-type and vaccine strains of canine distemper virus (CDV). A pair of primers (P1 and P4) specific for CDV corresponding to the highly conserved region of the CDV genome were used as a common primer pair in the first-round PCR of the nested PCR. Primers P2 specific for CDV wild-type strains, were used as the forward primer together with the common reverse primer P4 in the second round of nested PCR. Primers P3, P5 specific for CDV wild-type strain or vaccine strain, were used as the forward primer together with the common reverse primer P4+P6 in the second round of nested PCR. A fragment of 177 bp was amplified from vaccine strain genomic RNA, and a fragment of 247 bp from wild-type strain genomic RNA in the RT-nPCR, and two fragments of 247 bp and 177 bp were amplified from the mixed samples of vaccine and wild-type strains. No amplification was achieved for uninfected cells, or cells infected with Newcastle disease virus (NDV), canine parvovirus (CPV), canine coronavirus (CCV), rabies virus (RV), or canine adenovirus (CAV). The RT-nPCR method was used to detect 30 field samples suspected of canine distemper from Heilongjiang and Jilin Provinces, and 51 samples in Shandong province. As a result of 30 samples, were found to be wild-type-like, and 5 to be vaccine-strain-like. The RT-nPCR method can be used to effectively detect and differentiate wild-type CDV-infected dogs from dogs vaccinated with CDV vaccine, and thus can be used in clinical detection and epidemiological surveillance. Date A point or period of time associated with an event in the lifecycle of the resource 2010 Identifier An unambiguous reference to the resource within a given context DOI: 10.1186/1743-422X-7-86 Source A related resource from which the described resource is derived Virology Journal Publisher An entity responsible for making the resource available BMC Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Infectious and parasitic diseases Language A language of the resource EN