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                  <text>Coronavirus</text>
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                  <text>Dominio científico: Coronavirus</text>
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                <text>A single dose of recombinant VSV-∆G-spike vaccine provides protection against SARS-CoV-2 challenge</text>
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                <text>Yfat Yahalom-Ronen, Hadas Tamir, Sharon Melamed, Boaz Politi, Ohad Shifman, Hagit Achdout, Einat B. Vitner, Ofir Israeli, Elad Milrot, Dana Stein, Inbar Cohen-Gihon, Shlomi Lazar, Hila Gutman, Itai Glinert, Lilach Cherry, Yaron Vagima, Shirley Lazar, Shay Weiss, Amir Ben-Shmuel, Roy Avraham, Reut Puni, Edith Lupu, Elad Bar-David, Assa Sittner, Noam Erez, Ran Zichel, Emanuelle Mamroud, Ohad Mazor, Haim Levy, Orly Laskar, Shmuel Yitzhaki, Shmuel C. Shapira, Anat Zvi, Adi Beth-Din, Nir Paran, Tomer Israely</text>
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                <text>Here, the authors generate a replication-competent VSV based vaccine expressing SARS-CoV-2 spike protein and show protection in the hamster model with one dose. Analysis of the antibody response in mice shows induction of neutralizing antibodies and suggests a desirable Th1-biased response to the vaccine.</text>
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                <text>2020</text>
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                <text>10.1038/s41467-020-20228-7</text>
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                <text>Epidemiology and Health</text>
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                <text>Korean Society of Epidemiology</text>
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              <name>Title</name>
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                  <text>Coronavirus</text>
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              <description>An account of the resource</description>
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                  <text>Dominio científico: Coronavirus</text>
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                <text>A single polar residue and distinct membrane topologies impact the function of the infectious bronchitis coronavirus E protein.</text>
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                <text>Travis R. Ruch, Carolyn E. Machamer</text>
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                <text>The coronavirus E protein is a small membrane protein with a single predicted hydrophobic domain (HD), and has a poorly defined role in infection. The E protein is thought to promote virion assembly, which occurs in the Golgi region of infected cells. It has also been implicated in the release of infectious particles after budding. The E protein has ion channel activity in vitro, although a role for channel activity in infection has not been established. Furthermore, the membrane topology of the E protein is of considerable debate, and the protein may adopt more than one topology during infection. We previously showed that the HD of the infectious bronchitis virus (IBV) E protein is required for the efficient release of infectious virus, an activity that correlated with disruption of the secretory pathway. Here we report that a single residue within the hydrophobic domain, Thr16, is required for secretory pathway disruption. Substitutions of other residues for Thr16 were not tolerated. Mutations of Thr16 did not impact virus assembly as judged by virus-like particle production, suggesting that alteration of secretory pathway and assembly are independent activities. We also examined how the membrane topology of IBV E affected its function by generating mutant versions that adopted either a transmembrane or membrane hairpin topology. We found that a transmembrane topology was required for disrupting the secretory pathway, but was less efficient for virus-like particle production. The hairpin version of E was unable to disrupt the secretory pathway or produce particles. The findings reported here identify properties of the E protein that are important for its function, and provide insight into how the E protein may perform multiple roles during infection.</text>
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                <text>2012</text>
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                <text>DOI: 10.1371/journal.ppat.1002674</text>
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                <text>PLoS Pathogens</text>
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                <text>Public Library of Science (PLoS)</text>
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                <text>Biology (General), Immunologic diseases. Allergy</text>
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                <text>EN</text>
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                  <text>Coronavirus</text>
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              <description>An account of the resource</description>
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                  <text>Dominio científico: Coronavirus</text>
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      <name>Text</name>
      <description>A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.</description>
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                <text>A single-cell and spatial atlas of autopsy tissues reveals pathology and cellular targets of SARS-CoV-2.</text>
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            <description>An entity primarily responsible for making the resource</description>
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                <text>Katherine J Siddle, Carly G K Ziegler, Toni M Delorey, Graham Heimberg, Rachelly Normand, Yiming Yang, Asa Segerstolpe, Domenic Abbondanza, Stephen J Fleming, Ayshwarya Subramanian, Daniel T Montoro, Karthik A Jagadeesh, Kushal K Dey, Pritha Sen, Michal Slyper, Yered H Pita-Juárez, Devan Phillips, Zohar Bloom-Ackerman, Nick Barkas, Andrea Ganna, James Gomez, Erica Normandin, Pourya Naderi, Yury V Popov, Siddharth S Raju, Sebastian Niezen, Linus T-Y Tsai, Malika Sud, Victoria M Tran, Shamsudheen K Vellarikkal, Liat Amir-Zilberstein, Deepak S Atri, Joseph Beechem, Olga R Brook, Jonathan Chen, Prajan Divakar, Phylicia Dorceus, Jesse M Engreitz, Adam Essene, Donna M Fitzgerald, Robin Fropf, Steven Gazal, Joshua Gould, John Grzyb, Tyler Harvey, Jonathan Hecht, Tyler Hether, Judit Jane-Valbuena, Michael Leney-Greene, Hui Ma, Cristin McCabe, Daniel E McLoughlin, Eric M Miller, Christoph Muus, Mari Niemi, Robert Padera, Liuliu Pan, Deepti Pant, Carmel Pe'er, Jenna Pfiffner-Borges, Christopher J Pinto, Jacob Plaisted, Jason Reeves, Marty Ross, Melissa Rudy, Erroll H Rueckert, Michelle Siciliano, Alexander Sturm, Ellen Todres, Avinash Waghray, Sarah Warren, Shuting Zhang, Daniel R Zollinger, Lisa Cosimi, Rajat M Gupta, Nir Hacohen, Winston Hide, Alkes L Price, Jayaraj Rajagopal, Purushothama Rao Tata, Stefan Riedel, Gyongyi Szabo, Timothy L Tickle, Deborah Hung, Pardis C Sabeti, Richard Novak, Robert Rogers, Donald E Ingber, Z Gordon Jiang, Dejan Juric, Mehrtash Babadi, Samouil L Farhi, James R Stone, Ioannis S Vlachos, Isaac H Solomon, Orr Ashenberg, Caroline B M Porter, Bo Li, Alex K Shalek, Alexandra-Chloé Villani, Orit Rozenblatt-Rosen, Aviv Regev</text>
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            <description>An account of the resource</description>
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                <text>The SARS-CoV-2 pandemic has caused over 1 million deaths globally, mostly due to acute lung injury and acute respiratory distress syndrome, or direct complications resulting in multiple-organ failures. Little is known about the host tissue immune and cellular responses associated with COVID-19 infection, symptoms, and lethality. To address this, we collected tissues from 11 organs during the clinical autopsy of 17 individuals who succumbed to COVID-19, resulting in a tissue bank of approximately 420 specimens. We generated comprehensive cellular maps capturing COVID-19 biology related to patients' demise through single-cell and single-nucleus RNA-Seq of lung, kidney, liver and heart tissues, and further contextualized our findings through spatial RNA profiling of distinct lung regions. We developed a computational framework that incorporates removal of ambient RNA and automated cell type annotation to facilitate comparison with other healthy and diseased tissue atlases. In the lung, we uncovered significantly altered transcriptional programs within the epithelial, immune, and stromal compartments and cell intrinsic changes in multiple cell types relative to lung tissue from healthy controls. We observed evidence of: alveolar type 2 (AT2) differentiation replacing depleted alveolar type 1 (AT1) lung epithelial cells, as previously seen in fibrosis; a concomitant increase in myofibroblasts reflective of defective tissue repair; and, putative TP63 + intrapulmonary basal-like progenitor (IPBLP) cells, similar to cells identified in H1N1 influenza, that may serve as an emergency cellular reserve for severely damaged alveoli. Together, these findings suggest the activation and failure of multiple avenues for regeneration of the epithelium in these terminal lungs. SARS-CoV-2 RNA reads were enriched in lung mononuclear phagocytic cells and endothelial cells, and these cells expressed distinct host response transcriptional programs. We corroborated the compositional and transcriptional changes in lung tissue through spatial analysis of RNA profiles in situ and distinguished unique tissue host responses between regions with and without viral RNA, and in COVID-19 donor tissues relative to healthy lung. Finally, we analyzed genetic regions implicated in COVID-19 GWAS with transcriptomic data to implicate specific cell types and genes associated with disease severity. Overall, our COVID-19 cell atlas is a foundational dataset to better understand the biological impact of SARS-CoV-2 infection across the human body and empowers the identification of new therapeutic interventions and prevention strategies.</text>
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                <text>2021</text>
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            <name>Identifier</name>
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              <elementText elementTextId="75108">
                <text>10.1101/2021.02.25.430130</text>
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                <text>bioRxiv : the preprint server for biology</text>
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              <name>Title</name>
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                  <text>Coronavirus</text>
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                  <text>Dominio científico: Coronavirus</text>
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      <name>Text</name>
      <description>A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.</description>
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            <name>Title</name>
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                <text>A Single-Center Experience with Lung Transplants During the COVID-19 Pandemic.</text>
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            <name>Creator</name>
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                <text>Wojtek Karolak, Elżbieta Woźniak-Grygiel, Monika Łącka, Jacek Wojarski, Salma Ali Addo, Adith Kumaravel, Salin Khan, Reema Shinde, Ikram Mukhtar Nuur, Feven Kifle Reta, Jan Rogowski, Ewa Jassem, Krystyna Pastwa, Aleksandra Pałasz, Nikodem Ulatowski, Sławomir Żegleń</text>
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                <text>BACKGROUND This single-center study analyzed distinctions between lung transplants performed in the Department of Cardiac and Vascular surgery of the University Clinical Center in Gdańsk, Poland before and during the COVID-19 pandemic. MATERIAL AND METHODS There were 189 patients who underwent the qualification procedure to lung transplantation in the Department of Cardiac and Vascular Surgery of the University Clinical Center in Gdańsk, Poland in the years 2019 and 2020. The control group consisted of 12 patients transplanted in 2019, and the study group consisted of 16 patients transplanted in 2020. RESULTS During 2019, the qualification process was performed in 102 patients with pulmonary end-stage diseases. In 2020, despite the 3-month lockdown related to organizational changes in the hospital, 87 qualification processes were performed. The mortality rate of patients on the waiting list in 2020 was 14.3% (6 patients died), and during 2019 the rate was also 14.3% (4 patients died). Donor qualifications were according to ISHLT criteria. The distribution of donors in both years was similar. There was no relationship between the geographic area of residence and source of donors. In 2019, all 12 patients had double-lung transplant. In 2020, 11 patients had double-lung transplant and 5 patients had single-lung transplant. There was no difference in ventilation time and PGD aside from a shorter ICU stay in 2020. CONCLUSIONS Lung transplants were relatively well-conducted despite the continued obstacles of the COVID-19 pandemic.</text>
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                <text>2021</text>
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            <name>Identifier</name>
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                <text>10.12659/AOT.929946</text>
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                <text>Annals of transplantation</text>
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                  <text>Dominio científico: Coronavirus</text>
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        <description>The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.</description>
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          <element elementId="50">
            <name>Title</name>
            <description>A name given to the resource</description>
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              <elementText elementTextId="24971">
                <text>A single-center, retrospective study of COVID-19 features in children: a descriptive investigation</text>
              </elementText>
            </elementTextContainer>
          </element>
          <element elementId="39">
            <name>Creator</name>
            <description>An entity primarily responsible for making the resource</description>
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              <elementText elementTextId="24972">
                <text>Wei Chen, Hui Li, Jie Tian, Jun Xia, Xi Zhou, Rafael Ramos, Huijing Ma, Jiani Hu, Jianbo Shao, Maxwell Thomas Laws, Luke David Wesemann, Baiqi Zhu</text>
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          <element elementId="41">
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                <text>Abstract Background Compared to adults, there are relatively few studies on COVID-19 infection in children, and even less focusing on the unique features of COVID-19 in children in terms of laboratory findings, locations of computerized tomography (CT) lesions, and the role of CT in evaluating clinical recovery. The objective of this study is to report the results from patients at Wuhan Children’s Hospital, located within the initial center of the outbreak. Methods Clinical, imaging, and laboratory data of 76 children were collected retrospectively and analyzed with the Fisher exact test and Cox regression statistical methods. Results Among 50 children with a positive COVID-19 real-time reverse-transcriptase polymerase chain reaction (PCR), five had negative PCR results initially but showed positive results in subsequent tests. Eight (16%) patients had lymphopenia, seven (14%) with thrombocytopenia, four (8%) with lymphocytosis, two (4%) with thrombocytosis, ten (20%) with elevated C-reactive protein, four (8%) with hemoglobin above, and six (12%) with below standard reference values. Seven (14%) of the 50 had no radiologic evidence of disease on chest CT. For the 43 patients who had abnormal CT findings, in addition to previously reported patterns of ground-glass opacity (67%), local patchy shadowing (37%), local bilateral patchy shadowing (21%), and lesion location of lower lobes (65%), other CT features include that an overwhelming number of pediatric patients had lesions in the subpleural area (95%) and 22 of the 28 lower lobe lesions were in the posterior segment (78%). Lesions in most of the 15 patients (67%) who received chest CT at discharge were not completely absorbed, and 26% of these pediatric patients had CT lesions that were either unchanged or worse. Conclusions There were a few differences between COVID-19 children and COVID-19 adults in terms of laboratory findings and CT characteristics. CT is a powerful tool to detect and characterize COVID-19 pneumonia but has little utility in evaluating clinical recovery for children. These results oppose current COVID-19 hospital discharge criteria in China, as one requirement is that pulmonary imaging must show significant lesion absorption prior to discharge. These differences between pediatric and adult cases of COVID-19 may necessitate pediatric-specific discharge criteria.</text>
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            <elementTextContainer>
              <elementText elementTextId="24974">
                <text>2020</text>
              </elementText>
            </elementTextContainer>
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          <element elementId="49">
            <name>Subject</name>
            <description>The topic of the resource</description>
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              <elementText elementTextId="24975">
                <text>Epidemiology, Children, Pediatric, coronavirus, SARS-CoV-2, COVID-19</text>
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            </elementTextContainer>
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            <name>Identifier</name>
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              <elementText elementTextId="24976">
                <text>DOI: 10.1186/s12916-020-01596-9</text>
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          <element elementId="48">
            <name>Source</name>
            <description>A related resource from which the described resource is derived</description>
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              <elementText elementTextId="24977">
                <text>BMC Medicine</text>
              </elementText>
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              <elementText elementTextId="24978">
                <text>BMC</text>
              </elementText>
            </elementTextContainer>
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                <text>Medicine</text>
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            <element elementId="50">
              <name>Title</name>
              <description>A name given to the resource</description>
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                  <text>Coronavirus</text>
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              </elementTextContainer>
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              <name>Description</name>
              <description>An account of the resource</description>
              <elementTextContainer>
                <elementText elementTextId="2">
                  <text>Dominio científico: Coronavirus</text>
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          <element elementId="50">
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              <elementText elementTextId="36192">
                <text>A Snapshot of the Global Race for Vaccines Targeting SARS-CoV-2 and the COVID-19 Pandemic</text>
              </elementText>
            </elementTextContainer>
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          <element elementId="39">
            <name>Creator</name>
            <description>An entity primarily responsible for making the resource</description>
            <elementTextContainer>
              <elementText elementTextId="36193">
                <text>Colin D. Funk, Craig Laferriere, Ali Ardakani</text>
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            </elementTextContainer>
          </element>
          <element elementId="41">
            <name>Description</name>
            <description>An account of the resource</description>
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              <elementText elementTextId="36194">
                <text>A novel coronavirus SARS-CoV-2 causing Coronavirus disease 2019 (COVID-19) has entered the human population and has spread rapidly around the world in the first half of 2020 causing a global pandemic. The virus uses its spike glycoprotein receptor-binding domain to interact with host cell angiotensin-converting enzyme 2 (ACE2) sites to initiate a cascade of events that culminate in severe acute respiratory syndrome in some individuals. In efforts to curtail viral spread, authorities initiated far-reaching lockdowns that have disrupted global economies. The scientific and medical communities are mounting serious efforts to limit this pandemic and subsequent waves of viral spread by developing preventative vaccines and repurposing existing drugs as potential therapies. In this review, we focus on the latest developments in COVID-19 vaccine development, including results of the first Phase I clinical trials and describe a number of the early candidates that are emerging in the field. We seek to provide a balanced coverage of the seven main platforms used in vaccine development that will lead to a desired target product profile for the “ideal” vaccine. Using tales of past vaccine discovery efforts that have taken many years or that have failed, we temper over exuberant enthusiasm with cautious optimism that the global medical community will reach the elusive target to treat COVID-19 and end the pandemic.</text>
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            <name>Date</name>
            <description>A point or period of time associated with an event in the lifecycle of the resource</description>
            <elementTextContainer>
              <elementText elementTextId="36195">
                <text>2020</text>
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          <element elementId="49">
            <name>Subject</name>
            <description>The topic of the resource</description>
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              <elementText elementTextId="36196">
                <text>vaccine, immune response, coronavirus, clinical trial, SARS-CoV-2, COVID-19</text>
              </elementText>
            </elementTextContainer>
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            <name>Identifier</name>
            <description>An unambiguous reference to the resource within a given context</description>
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              <elementText elementTextId="36197">
                <text>DOI: 10.3389/fphar.2020.00937</text>
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            </elementTextContainer>
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          <element elementId="48">
            <name>Source</name>
            <description>A related resource from which the described resource is derived</description>
            <elementTextContainer>
              <elementText elementTextId="36198">
                <text>Frontiers in Pharmacology</text>
              </elementText>
            </elementTextContainer>
          </element>
          <element elementId="45">
            <name>Publisher</name>
            <description>An entity responsible for making the resource available</description>
            <elementTextContainer>
              <elementText elementTextId="36199">
                <text>Frontiers Media S.A.</text>
              </elementText>
            </elementTextContainer>
          </element>
          <element elementId="38">
            <name>Coverage</name>
            <description>The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant</description>
            <elementTextContainer>
              <elementText elementTextId="36200">
                <text>Therapeutics. Pharmacology</text>
              </elementText>
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                  <text>Agricultura sostenible</text>
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              <name>Description</name>
              <description>An account of the resource</description>
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                  <text>Dominio científico: Agricultura sostenible</text>
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              </elementTextContainer>
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        <name>Dublin Core</name>
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          <element elementId="50">
            <name>Title</name>
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              <elementText elementTextId="152889">
                <text>A Soberania Alimentar e o Programa Nacional de Alimentação Escolar no município de Piracicaba (SP) – concepções e redefinições</text>
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            <description>An entity primarily responsible for making the resource</description>
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              <elementText elementTextId="152890">
                <text>Samira Gaiad Cibim de Camargo Bosquilia, Maria Angélica Penatti Pipitone</text>
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          <element elementId="41">
            <name>Description</name>
            <description>An account of the resource</description>
            <elementTextContainer>
              <elementText elementTextId="152891">
                <text>O conceito de segurança alimentar surgiu após a II Guerra Mundial trazendo à tona a formulação de critérios ditos como essenciais para uma alimentação digna e correta para todos. Tal conceito serviu de base para discussões e propostas de movimentos sociais que resultaram na formulação do conceito de Soberania Alimentar, na década de 1990. A Soberania Alimentar apregoa que todos os indivíduos, comunidades, povos e países possuem o direito de definir suas próprias políticas da agricultura, do trabalho, do alimento e da terra. A presente pesquisa teve por objetivo analisar o conceito de Soberania Alimentar e suas implicações no âmbito da gestão do Programa Nacional de Alimentação Escolar (PNAE) no município de Piracicaba (estado de São Paulo). Para isso, foram realizadas pesquisas bibliográficas, análise documental e entrevistas semiestruturadas. Entre os resultados, foi possível verificar que o conceito de Soberania Alimentar é pouco explorado no desenvolvimento do PNAE, ainda que seja encontrado nas falas dos gestores e nos documentos do programa. O maior desafio do PNAE, de acordo com a pesquisa, resulta na dificuldade de coordenação dos muitos atores envolvidos na gestão do PNAE em torno da adoção dos princípios e práticas da Soberania Alimentar.</text>
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            <name>Date</name>
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              <elementText elementTextId="152892">
                <text>2016</text>
              </elementText>
            </elementTextContainer>
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            <name>Subject</name>
            <description>The topic of the resource</description>
            <elementTextContainer>
              <elementText elementTextId="152893">
                <text>Soberania Alimentar. Alimentação Escolar. Agricultura Familiar</text>
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            <name>Identifier</name>
            <description>An unambiguous reference to the resource within a given context</description>
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              <elementText elementTextId="152894">
                <text>10.20396/san.v23i2.8647779</text>
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            <name>Source</name>
            <description>A related resource from which the described resource is derived</description>
            <elementTextContainer>
              <elementText elementTextId="152895">
                <text>Revista Segurança Alimentar e Nutricional</text>
              </elementText>
            </elementTextContainer>
          </element>
          <element elementId="45">
            <name>Publisher</name>
            <description>An entity responsible for making the resource available</description>
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              <elementText elementTextId="152896">
                <text>Universidade Estadual de Campinas</text>
              </elementText>
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                <text>Agriculture (General), Nutrition. Foods and food supply</text>
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                <text>&lt;a href="https://periodicos.sbu.unicamp.br/ojs/index.php/san/article/view/8647779" target="_blank" rel="noreferrer noopener"&gt;https://periodicos.sbu.unicamp.br/ojs/index.php/san/article/view/8647779&lt;/a&gt;</text>
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                  <text>Agricultura sostenible</text>
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                  <text>Dominio científico: Agricultura sostenible</text>
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                <text>A soberania do feijão</text>
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                <text>Rogério Luiz Backes</text>
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                <text>Revista Agropecuária Catarinense</text>
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                <text>Empresa de Pesquisa Agropecuária e Extensão Rural de Santa Catarina - Epagri</text>
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                <text>Agriculture (General)</text>
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                <text>&lt;a href="https://publicacoes.epagri.sc.gov.br/RAC/article/view/590" target="_blank" rel="noreferrer noopener"&gt;https://publicacoes.epagri.sc.gov.br/RAC/article/view/590&lt;/a&gt;</text>
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                  <text>Dominio científico: Coronavirus</text>
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      <name>Text</name>
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                <text>A social network analysis of the spread of COVID-19 in South Korea and policy implications</text>
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                <text>Myoungsoon You, Wonkwang Jo, Dukjin Chang, Ghi-Hoon Ghim</text>
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                <text>Abstract This study estimates the COVID-19 infection network from actual data and draws on implications for policy and research. Using contact tracing information of 3283 confirmed patients in Seoul metropolitan areas from January 20, 2020 to July 19, 2020, this study created an infection network and analyzed its structural characteristics. The main results are as follows: (i) out-degrees follow an extremely positively skewed distribution; (ii) removing the top nodes on the out-degree significantly decreases the size of the infection network, and (iii) the indicators that express the infectious power of the network change according to governmental measures. Efforts to collect network data and analyze network structures are urgently required for the efficiency of governmental responses to COVID-19. Implications for better use of a metric such as R0 to estimate infection spread are also discussed.</text>
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                <text>2021</text>
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                <text>10.1038/s41598-021-87837-0</text>
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                <text>The objective of study was to assess and evaluate factors that affect entrepreneurial activities carried out by formal and informal migrant entrepreneurs from Ethiopia who conduct business operations in the nine provinces of South Africa. The study was descriptive and exploratory in nature. The design of the study was descriptive and cross-sectional. Data were collected from a stratified random sample of 3,045 migrant entrepreneurs from Ethiopia who conduct business in the nine provinces of South Africa. Stratified random sampling was used for the selection of eligible entrepreneurs. The study found that about 76% of businesses operated by migrant entrepreneurs from Ethiopia were profitable, whereas the remaining 24% of businesses were not profitable. About 32% of entrepreneurs were attracted to South Africa due to better infrastructural facilities. About 25% of entrepreneurs were attracted to South Africa due to better socioeconomic conditions. About 78% of migrant entrepreneurs had conducted business in South Africa for five years or more at the time of data collection. About 34% of businesses paid tax to the South African Revenue Service (SARS) on a regular basis. About 38% of businesses employed at least one South African in their businesses. About 85% of entrepreneurs stated that they had good working relationships with members of the various local communities in South Africa.</text>
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                <text>economic analysis, Ethiopian migrant entrepreneurs, logit analysis, South Africa</text>
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