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                <text>Las bacterias endófitas residen en tejidos de las plantas, principalmente espacios intercelulares, raramente en espacios intracelulares y dentro de tejidos vasculares sin causar síntomas de enfermedad en la planta. Las discusiones sobre el origen de las bacterias endófitas y la forma de penetración, además de los mecanismos de colonización, consideran la hipótesis de que se originaron desde semillas, de la rizosfera, de microflora de filoplano o de material utilizado para la propagación vegetativa. La penetración en la planta puede ocurrir por estomas, heridas, áreas de emergencia de raíces laterales, siendo que estas bacterias pueden producir enzimas hidrolíticas capaces de degradar la pared celular de los vegetales. Estudios moleculares reciente sobre diversidad de bacterias endófitas han revelado una alta riqueza de filotipos, que promueven el crecimiento de las planta, suprimen fitopatógenos, ayudan a remover contaminantes, solubilizan fosfato y contribuyen a la asimilación biológica de nitrógeno. El objetivo de esta revisión es dar a conocer la evolución, ecología, diversidad, funcionalidad y técnicas fisiológicas y moleculares para el aislamiento de bacterias endófitas asociadas a tejidos de plantas.</text>
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                <text>Revista Colombiana de Ciencia Animal Recia</text>
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                <text>Universidad de Sucre</text>
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                <text>Agriculture (General), Agriculture, Animal culture</text>
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                <text>Bacterias endófitas: una alternativa biologica para el control de burkholderia glumae en el cultivo del arroz en colombia.</text>
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                <text>Las investigaciones más comunes en ecología microbiana, han sido orientadas a las interacciones microbio-planta desde el punto de vista simbiótico y de patogénico. Las bacterias endófitas colonizan el interior de los tejidos de las plantas, principalmente espacios intercelulares, raramente en espacios intracelulares y dentro de tejidos vasculares sin causar síntomas de enfermedad en la planta. La penetración en la planta puede ocurrir por estomas, heridas, áreas de emergencia de raíces laterales, siendo que estas bacterias pueden producir enzimas hidrolíticas capaces de degradar la pared celular de los vegetales. Estudios moleculares reciente sobre diversidad de bacterias endófitas han revelado una alta riqueza de filotipos, que promueven el crecimiento de las planta, suprimen fitopatógenos, ayudan a remover contaminantes, solubilizan fosfato y contribuyen a la asimilación biológica de nitrógeno.</text>
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                <text>El objetivo de este estudio fue evaluar in vitro la actividad fijadora de nitrógeno y solubilizadora de fosfato de bacterias endófitas de pasto colosoana (Bothriochloa pertusa). Se colectó la raíz, los tallos y las hojas del pasto en cuatro zonas agroecológicas del municipio de Corozal, Sucre, Colombia, durante el segundo semestre del año 2012. Cada tejido fue sometido a proceso de desinfección superficial. Se aislaron bacterias endófitas en medio de cultivo agar R2A, a partir de cada tejido se determinó la densidad poblacional (UFC/g de tejido), por conteo en superficie; la separación de morfotipos se realizó mediante la forma, color, tamaño y apariencia. La actividad fijadora de nitrógeno y solubilizadora de fosfato se evaluó in vitro sobre medios de cultivos específicos. Todos los resultados obtenidos fueron analizados utilizando el programa estadístico R. Hubo diferencias significativas para densidades poblacional de bacterias con respecto a tejido, con mayores valores en raíz (1,61 x 1010/g raíz), seguida de tallo (7,44 x 109/g tallo) y los valores más bajos se reportaron para hojas (5,42 x 109/g hoja). Se encontró significancia entre la densidad poblacional con relación a los factores de zona, fincas y tipo de tejido analizado. Los resultados de la identificación con kit API20E confirmaron la presencia de las bacterias endófitas Aeromonas salmonicida y Pasteurella pneumotropica con capacidad simultánea de solubilizar fosfatos y fijar nitrógeno.</text>
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                <text>2014</text>
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          <element elementId="49">
            <name>Subject</name>
            <description>The topic of the resource</description>
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              <elementText elementTextId="147216">
                <text>Bothriochloa pertusa, biofertilizantes para pasto, microorganismos endófitos</text>
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            <name>Identifier</name>
            <description>An unambiguous reference to the resource within a given context</description>
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                <text>10.15517/am.v25i2.15425</text>
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            <name>Source</name>
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                <text>Agronomía Mesoamericana</text>
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            <name>Publisher</name>
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                <text>Universidad de Costa Rica</text>
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                <text>Agriculture</text>
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            <description>A related resource</description>
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                <text>&lt;a href="https://revistas.ucr.ac.cr/index.php/agromeso/article/view/15425" target="_blank" rel="noreferrer noopener"&gt;https://revistas.ucr.ac.cr/index.php/agromeso/article/view/15425&lt;/a&gt;</text>
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              <description>An account of the resource</description>
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                  <text>Dominio científico: Coronavirus</text>
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                <text>What do We Learn from Covid?</text>
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            <description>An entity primarily responsible for making the resource</description>
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                <text>Alexander R. Cuthbert</text>
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            <name>Description</name>
            <description>An account of the resource</description>
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                <text>-</text>
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            <name>Date</name>
            <description>A point or period of time associated with an event in the lifecycle of the resource</description>
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                <text>2020</text>
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            <name>Identifier</name>
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                <text>10.24843/JRS.2020.v07.i02.p01</text>
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            <name>Source</name>
            <description>A related resource from which the described resource is derived</description>
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              <elementText elementTextId="64837">
                <text>Ruang-Space: Jurnal Lingkungan Binaan</text>
              </elementText>
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            <name>Publisher</name>
            <description>An entity responsible for making the resource available</description>
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              <elementText elementTextId="64838">
                <text>Universitas Udayana</text>
              </elementText>
            </elementTextContainer>
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            <name>Coverage</name>
            <description>The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant</description>
            <elementTextContainer>
              <elementText elementTextId="64839">
                <text>Aesthetics of cities. City planning and beautifying</text>
              </elementText>
            </elementTextContainer>
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            <element elementId="50">
              <name>Title</name>
              <description>A name given to the resource</description>
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                  <text>Coronavirus</text>
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              </elementTextContainer>
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            <element elementId="41">
              <name>Description</name>
              <description>An account of the resource</description>
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                  <text>Dominio científico: Coronavirus</text>
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    <itemType itemTypeId="1">
      <name>Text</name>
      <description>A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.</description>
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      <elementSet elementSetId="1">
        <name>Dublin Core</name>
        <description>The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.</description>
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            <description>A name given to the resource</description>
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                <text>Propagation, Inactivation, and Safety Testing of SARS-CoV-2</text>
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            <name>Creator</name>
            <description>An entity primarily responsible for making the resource</description>
            <elementTextContainer>
              <elementText elementTextId="81124">
                <text>Alexander S. Jureka, Jesus A. Silvas, Christopher F. Basler</text>
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          <element elementId="41">
            <name>Description</name>
            <description>An account of the resource</description>
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              <elementText elementTextId="81125">
                <text>In late 2019, a novel coronavirus, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) emerged in Wuhan, the capital of the Chinese province Hubei. Since then, SARS-CoV-2 has been responsible for a worldwide pandemic resulting in over 4 million infections and over 250,000 deaths. The pandemic has instigated widespread research related to SARS-CoV-2 and the disease that it causes, COVID-19. Research into this new virus will be facilitated by the availability of clearly described and effective procedures that enable the propagation and quantification of infectious virus. As work with the virus is recommended to be performed at biosafety level 3, validated methods to effectively inactivate the virus to enable the safe study of RNA, DNA, and protein from infected cells are also needed. Here, we report methods used to grow SARS-CoV-2 in multiple cell lines and to measure virus infectivity by plaque assay using either agarose or microcrystalline cellulose as an overlay as well as a SARS-CoV-2 specific focus forming assay. We also demonstrate effective inactivation by TRIzol, 10% neutral buffered formalin, beta propiolactone, and heat.</text>
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            <name>Date</name>
            <description>A point or period of time associated with an event in the lifecycle of the resource</description>
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                <text>2020</text>
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            <name>Subject</name>
            <description>The topic of the resource</description>
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                <text>coronavirus, virus, virology, SARS-CoV-2, plaque assay, inactivation</text>
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            </elementTextContainer>
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            <name>Identifier</name>
            <description>An unambiguous reference to the resource within a given context</description>
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              <elementText elementTextId="81128">
                <text>10.3390/v12060622</text>
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            <description>A related resource from which the described resource is derived</description>
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                <text>Epidemiology and Health</text>
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            <name>Publisher</name>
            <description>An entity responsible for making the resource available</description>
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              <elementText elementTextId="81130">
                <text>Korean Society of Epidemiology</text>
              </elementText>
            </elementTextContainer>
          </element>
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            <description>The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant</description>
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              <elementText elementTextId="81131">
                <text>Microbiology</text>
              </elementText>
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              <name>Title</name>
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                  <text>Coronavirus</text>
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            <element elementId="41">
              <name>Description</name>
              <description>An account of the resource</description>
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                  <text>Dominio científico: Coronavirus</text>
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      <name>Text</name>
      <description>A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.</description>
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      <elementSet elementSetId="1">
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            <name>Title</name>
            <description>A name given to the resource</description>
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                <text>Features of case management with neuromuscular disease during COVID-19. Clinical impression</text>
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            <name>Creator</name>
            <description>An entity primarily responsible for making the resource</description>
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              <elementText elementTextId="47575">
                <text>Alexander S. Samoylov, Olga V. Karpova, Yury D. Udalov, Roman A. Kudryavtsev</text>
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            <description>An account of the resource</description>
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                <text>The new COVID-19 coronavirus infection, which has become a pandemic, is a very dangerous disease, the clinical picture of which can vary from mild to extremely severe forms of the course. Currently, there are no complete data on the pathogenetic mechanism of SARS-CoV-2, but there are extensive data on the probable risk factors for the development of extremely severe forms of COVID-19. The study of such factors becomes most suitable in terms of preventing their development and influence on the course of the disease in individuals with compromised immune systems and patients with impaired neuromuscular transmission. The article describes two clinical cases of extremely severe COVID-19 in patients with impaired neuromuscular transmission. Based on the analysis of the course of diseases, the conclusions are made about the possible aggravation and mutual activation of the immunopathological process with the launch of the cascade mechanism of the cytokine storm. An assumption has been made about the influence of human leukocyte antigen (HLA) on the severity of COVID-19, which is confirmed by a positive dynamics against the background of administration of IVIG, glucocorticosteroids (GCS), virusinactivated plasma and extracorporeal detoxification methods.</text>
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                <text>2020</text>
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                <text>coronavirus, Viral pneumonia, covid-19, severe disease, Myasthenia, critical illness polyneuropathy, impaired neuromuscular transmission</text>
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            <description>An unambiguous reference to the resource within a given context</description>
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                <text>10.17816/clinpract34580</text>
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                <text>Клиническая практика</text>
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                <text>Eco-vector</text>
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            <description>The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant</description>
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                <text>Medicine</text>
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              <name>Title</name>
              <description>A name given to the resource</description>
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                  <text>Coronavirus</text>
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                  <text>Dominio científico: Coronavirus</text>
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      <description>A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.</description>
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            <description>A name given to the resource</description>
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                <text>A clinical case of successful application of a new treatment method for severe COVID-19</text>
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              <elementText elementTextId="83359">
                <text>Alexander S. Samoylov, Yuri D. Udalov, Nikolai M. Kruglyakov, Dmitry А. Terekhov, German I. Bagzhanov, Sergey S. Ochkin</text>
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            <description>An account of the resource</description>
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                <text>COVID-19, formerly coronavirus infection 2019-nCoV, is a potentially severe acute respiratory infection caused by the SARS-CoV-2 coronavirus (2019-nCoV). It is a dangerous disease that can occur both in the form of a mild acute respiratory viral infection and in a severe form with the development of bilateral polysegmental viral pneumonia, specific complications of which may include acute respiratory distress syndrome, resulting in the respiratory failure with a high risk of death. Due to the absence of etiotropic therapy for the coronavirus infection, pathogentic treatment becomes of primary importance. We present a clinical case of a severe form of COVID-19 in a 33-year-old man to whom we administered a new method of pathogenetic treatment of this disease based on immunosuppresiive therapy followed by therapeutic plasma exchange and infusion of non-specific human immunoglobulin.</text>
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                <text>covid-19, Case report, immunosuppression, plasma exchange, selective plasma filtration</text>
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                <text>10.17816/clinpract34529</text>
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                <text>Клиническая практика</text>
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                <text>Eco-vector</text>
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                <text>Medicine</text>
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                  <text>Agricultura sostenible</text>
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                <text>Empatía y percepción del riesgo del cambio climático en estudiantes de Ciencias de la Salud</text>
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                <text>Alexander Salazar Ceballos, Lidice Alvarez-Miño</text>
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                <text>Para determinar el aumento de los niveles de empatía y percepción del riesgo del cambio climático en estudiantes universitarios de la salud se realizó un estudio cuasi-experimental. La intervención fue una estrategia educativa. Se seleccionaron por conveniencia dos grupos de estudiantes de ciencias de la salud. Los niveles de empatía se midieron con la Escala de Empatía Médica de Jefferson versión estudiantes y de la Encuesta de percepción sobre riesgo al cambio climático como una amenaza para la salud humana se midieron dos ítems: índice de percepción de riesgo y conocimiento sobre los efectos en la salud por causa del cambio climático. Se encontraron diferencias entre pre y post-test (p &lt; 0,05). En el pre-test ambos grupos presentaron niveles de empatía altos con un promedio de 107. Para el post-test, el grupo experimental, presentó un aumento estadísticamente significativo en los niveles de empatía. Se establecieron correlaciones significativas entre empatía global y factores de empatía: cuidado con compasión y toma de perspectiva; y correlación significativa entre el factor de empatía Cuidado con compasión y Conocimiento sobre los efectos en la salud por el cambio climático. Este trabajo evidenció como una estrategia educativa puede fomentar la empatía en estudiantes de la salud.</text>
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                <text>2020</text>
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                <text>Cambio climático, Estudiantes del Área de la Salud, empatia, estudios controlados antes y después, salud pública</text>
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            <name>Identifier</name>
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                <text>10.21676/2389783X.3317</text>
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                <text>Duazary</text>
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            <name>Publisher</name>
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                <text>Universidad del Magdalena</text>
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            <name>Coverage</name>
            <description>The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant</description>
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                <text>Medicine, Medicine (General)</text>
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            <name>Relation</name>
            <description>A related resource</description>
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                <text>&lt;a href="http://revistas.unimagdalena.edu.co/index.php/duazary/article/view/3317" target="_blank" rel="noreferrer noopener"&gt;http://revistas.unimagdalena.edu.co/index.php/duazary/article/view/3317&lt;/a&gt;</text>
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