Antiviral activity of a Bacillus sp: P34 peptide against pathogenic viruses of domestic animals

Antiviral activity of a Bacillus sp: P34 peptide against pathogenic viruses of domestic animals

Débora Scopel e Silva, Clarissa Caetano de Castro, Fábio da Silva e Silva, Voltaire Sant'Anna, Gilberto D'Ávila Vargas, Marcelo de Lima, Geferson Fischer, Adriano Brandelli, Amanda de Souza da Motta, Silvia de Oliveira Hübner

P34 is an antimicrobial peptide produced by a Bacillus sp. strain isolated from the intestinal contents of a fish in the Brazilian Amazon basin with reported antibacterial activity. The aim of this work was to evaluate the peptide P34 for its in vitro antiviral properties against canine adenovirus type 2 (CAV-2), canine coronavirus (CCoV), canine distemper virus (CDV), canine parvovirus type 2 (CPV-2), equine arteritis virus (EAV), equine influenza virus (EIV), feline calicivirus (FCV) and feline herpesvirus type 1 (FHV-1). The results showed that the peptide P34 exhibited antiviral activity against EAV and FHV-1. The peptide P34 inhibited the replication of EAV by 99.9% and FHV-1 by 94.4%. Virucidal activity was detected only against EAV. When P34 and EAV were incubated for 6 h at 37 °C the viral titer reduced from 10(4.5) TCID50 to 10(2.75) TCID50, showing a percent of inhibition of 98.6%. In conclusion, our results demonstrated that P34 inhibited EAV and FHV-1 replication in infected cell cultures and it showed virucidal activity against EAV. Since there is documented resistance to the current drugs used against herpesviruses and there is no treatment for equine viral arteritis, it is advisable to search for new antiviral compounds to overcome these infections.

2014

antimicrobial peptides, antiviral activity, herpesvírus, equine viral arteritis

DOI: 10.1590/S1517-83822014000300043

Brazilian Journal of Microbiology

Sociedade Brasileira de Microbiologia

Microbiology

EN