https://socictopen.socict.org/files/original/f89bb5ef9cab2bd849993835a73385ca.pdf ed597eec07f7303ac7438366ef342ce7 Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Coronavirus Description An account of the resource Dominio científico: Coronavirus Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Unique <i>k</i>-mers as Strain-Specific Barcodes for Phylogenetic Analysis and Natural Microbiome Profiling Creator An entity primarily responsible for making the resource Valery V. Panyukov, Sergey S. Kiselev, Olga N Ozoline Description An account of the resource The need for a comparative analysis of natural metagenomes stimulated the development of new methods for their taxonomic profiling. Alignment-free approaches based on the search for marker k-mers turned out to be capable of identifying not only species, but also strains of microorganisms with known genomes. Here, we evaluated the ability of genus-specific k-mers to distinguish eight phylogroups of Escherichia coli (A, B1, C, E, D, F, G, B2) and assessed the presence of their unique 22-mers in clinical samples from microbiomes of four healthy people and four patients with Crohn&#8217;s disease. We found that a phylogenetic tree inferred from the pairwise distance matrix for unique 18-mers and 22-mers of 124 genomes was fully consistent with the topology of the tree, obtained with concatenated aligned sequences of orthologous genes. Therefore, we propose strain-specific &#8220;barcodes&#8221; for rapid phylotyping. Using unique 22-mers for taxonomic analysis, we detected microbes of all groups in human microbiomes; however, their presence in the five samples was significantly different. Pointing to the intraspecies heterogeneity of E. coli in the natural microflora, this also indicates the feasibility of further studies of the role of this heterogeneity in maintaining population homeostasis. Date A point or period of time associated with an event in the lifecycle of the resource 2020 Subject The topic of the resource bacterial genomes, genome barcodes, <i>k</i>-mers, Alignment-free algorithms, Phylogenetic trees, metagenomes, Taxonomic profiling, Phylotyping, Human microbiome Identifier An unambiguous reference to the resource within a given context DOI: 10.3390/ijms21030944 Source A related resource from which the described resource is derived International Journal of Molecular Sciences Publisher An entity responsible for making the resource available MDPI AG Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Biology (General), Chemistry Language A language of the resource EN