Isolation of Chavibetol and Methyleugenol from Essential Oil of <i>Pimenta pseudocaryophyllus</i> by High Performance Liquid Chromatography

Isolation of Chavibetol and Methyleugenol from Essential Oil of <i>Pimenta pseudocaryophyllus</i> by High Performance Liquid Chromatography

Edenilson dos Santos Niculau, Leandro do Prado Ribeiro, Thiago Felipe Ansante, João Batista Fernandes, Moacir Rossi Forim, Paulo Cezar Vieira, José Djair Vendramim, Maria Fátima das Graças Fernandes da Silva

A high performance liquid chromatography (HPLC) method was developed for the simultaneous isolation, on a semi-preparative scale, of chavibetol and methyleugenol from the crude essential oil of P. pseudocaryophyllus leaves. The purity of the isolated compounds and their quantifications were developed using GC/FID. Chavibetol was isolated with high purity (98.7%) and mass recovery (94.6%). The mass recovery (86.4%) and purity (85.3%) of methyleugenol were lower than those of chavibetol. Both compounds were identified on the basis of spectral analysis. The results suggest that the method can provide chavibetol with high purity, mass recovery, and productivity from crude essential, which will be used in bioassays against stored insect pests.

2018

GC/MS, HPLC isolation, Natural products, chavibetol, essential oil, methyl eugenol

10.3390/molecules23112909

Molecules

MDPI AG

Organic chemistry