https://socictopen.socict.org/files/original/68d261b4f7cf003f95f28ebcfbad50ed.pdf 5c22959c7e9eacd38c43465e6213bc7e Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Agricultura sostenible Description An account of the resource Dominio científico: Agricultura sostenible Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Identificación de polimorfismos en RXam2, un gen candidato de resistencia a la bacteriosis vascular de yuca Creator An entity primarily responsible for making the resource Elízabeth Contreras, Camilo López Description An account of the resource Título en ingles: Identification of polymorphisms in RXam2 a cassava bacterial blight resistance gene candidate Resumen La yuca se constituye en la base de la alimentación para más de mil millones de personas en el mundo. Una de las principales enfermedades de la yuca y que podría comprometer la seguridad alimentaria es la bacteriosis vascular ocasionada por la bacteria Xanthomonas axonopodis pv. manihotis (Xam). El gen candidato de resistencia RXam2 codifica para una proteína con dominios NBS (Nucleotide Binding Site) y LRR (Leucine Rich Repeats) y colocaliza con un QTL (Quantitative Trait Loci) que explica el 61.6% de la resistencia a la cepa CIO151 de Xam. En este trabajo se secuenció parcialmente el gen RXam2 en tres variedades de yuca: MCOL2246, TMS60444 y SG107-35 con el objetivo de tener una visión preliminar del grado de polimorfismos tipo SNP (Single Nucleotide Polymorphism) que se presenta en este gen. La región secuenciada incluye 507 pb de la región promotora y 1309 pb de la secuencia codificante. Se logró identificar 5 y 31 SNPs al interior de las variedades MCOL2246 y TMS60444, respectivamente. Al mismo tiempo el número de SNPs entre las variedades fue de 44, 34 y 23 para MCOL2246-TMS60444, TMS60444-SG107-35 y MCOL2246-SG107-35, respectivamente. El mayor número de SNPs estuvieron localizados en la región -500 a -300 pb que corresponden a un fragmento de la región promotora del gen, aunque también se identificó un importante número de polimorfismos en la región codificante. Este estudio permitirá identificar el número de polimorfismos en este gen en un mayor grupo de variedades de yuca con el fin de asociar estos polimorfismos con el fenotipo de resistencia/susceptibilidad. Palabras clave: yuca; resistencia; polimorfismos; SNP.   Abstract Cassava is a staple food for more than a billion people. One of the major diseases of cassava which could compromise food security is known as cassava bacterial blight, caused by the bacterium Xanthomonas axonopodis pv. manihotis (Xam). The RXam2 resistance gene candidate encodes a protein with Nucleotide Binding Site and Leucine Rich Repeats domains, and colocalizes with a QTL (Quantitative Trait Loci) that accounts for 61.6% of the resistance to the Xam strain CIO151. In this work we sequenced 1816 bp corresponding to a partial sequence of this gene in three cassava varieties with the aim of having a preliminary overview of the degree of Single Nucleotide Polymorphisms (SNPs). The sequenced regions included 507 bp of the promotor and 1309 bp of the coding sequence. It was possible to identify five and 31 intravarietal SNPs in MCOL2246 and TMS60444, respectively. In addition, the number of SNPs between varieties was 44, 34 and 23 for MCOL2246-TMS60444, TMS60444-SG107-35 and MCOL2246-SG107-35, respectively. The largest number of SNPs was located in the promoter region -500 to -300 bp. This study may help to develop alternatives to identify SNPs in a more diverse group of cassava varieties, and possibly associate them with resistance/susceptible phenotypes. Key words: Cassava; resistance; polymorphisms; SNP. Date A point or period of time associated with an event in the lifecycle of the resource 2011 Subject The topic of the resource Polymorphisms, SNP, Yuca, cassava, polimorfismos, resistance, resistencia Source A related resource from which the described resource is derived Revista Colombiana de Biotecnología Publisher An entity responsible for making the resource available Universidad Nacional de Colombia Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Biotechnology Relation A related resource <a href="https://revistas.unal.edu.co/index.php/biotecnologia/article/view/27918" target="_blank" rel="noreferrer noopener">https://revistas.unal.edu.co/index.php/biotecnologia/article/view/27918</a>