https://socictopen.socict.org/files/original/b2961ee643632ebb78864a12545f5b6c.pdf 21d3f6abde2a640debd634f4afb1da38 Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Coronavirus Description An account of the resource Dominio científico: Coronavirus Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource The Human Sodium Iodide Symporter as a Reporter Gene for Studying Middle East Respiratory Syndrome Coronavirus Pathogenesis Creator An entity primarily responsible for making the resource Svetlana Chefer, Jurgen Seidel, Adam S. Cockrell, Boyd Yount, Jeffrey Solomon, Katie R. Hagen, David X. Liu, Louis M. Huzella, Mia R. Kumar, Elena Postnikova, J. Kyle Bohannon, Matthew G. Lackemeyer, Kurt Cooper, Ariel Endlich-Frazier, Heema Sharma, David Thomasson, Christopher Bartos, Philip J. Sayre, Amy Sims, Julie Dyall, Michael R Holbrook, Peter B. Jahrling, Ralph S. Baric, Reed F. Johnson Description An account of the resource Advanced medical imaging such as single photon emission computed tomography with computed tomography (SPECT/CT) enhances fields such as oncology and cardiology. Application of SPECT/CT, magnetic resonance imaging, and positron emission tomography to infectious disease may enhance pathogenesis studies and provide alternate biomarkers of disease progression. The experiments described in this article focus on insertion of a SPECT/CT-compatible reporter gene into MERS-CoV to demonstrate that a functional SPECT/CT reporter gene can be inserted into a virus.Single photon emission computed tomography (SPECT) is frequently used in oncology and cardiology to evaluate disease progression and/or treatment efficacy. Such technology allows for real-time evaluation of disease progression and when applied to studying infectious diseases may provide insight into pathogenesis. Insertion of a SPECT-compatible reporter gene into a virus may provide insight into mechanisms of pathogenesis and viral tropism. The human sodium iodide symporter (hNIS), a SPECT and positron emission tomography reporter gene, was inserted into Middle East respiratory syndrome coronavirus (MERS-CoV), a recently emerged virus that can cause severe respiratory disease and death in afflicted humans to obtain a quantifiable and sensitive marker for viral replication to further MERS-CoV animal model development. The recombinant virus was evaluated for fitness, stability, and reporter gene functionality. The recombinant and parental viruses demonstrated equal fitness in terms of peak titer and replication kinetics, were stable for up to six in vitro passages, and were functional. Further in vivo evaluation indicated variable stability, but resolution limits hampered in vivo functional evaluation. These data support the further development of hNIS for monitoring infection in animal models of viral disease. Date A point or period of time associated with an event in the lifecycle of the resource 2018 Subject The topic of the resource MERS, coronavirus, medical imaging, Reporter gene Identifier An unambiguous reference to the resource within a given context DOI: 10.1128/mSphere.00540-18 Source A related resource from which the described resource is derived mSphere Publisher An entity responsible for making the resource available American Society for Microbiology Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Microbiology Language A language of the resource EN