https://socictopen.socict.org/files/original/5732fac717483ae53f8441ecd516ef92.pdf 24734bfe73e0f6eb42e8fd1fbd83c6ea Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Agricultura sostenible Description An account of the resource Dominio científico: Agricultura sostenible Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Búsqueda de genes de resistencia al pasador del fruto en poblaciones segregantes de tomate Search for genes for resistance to tomato fruit borer Creator An entity primarily responsible for making the resource Franco Alirio Vallejo Cabrera, Jhon Fredy Betancur Pérez, Karina López López, Juan Carlos Vaca-Vaca, Diana Milena Rodríguez-Mora Description An account of the resource Las estrategias de mejoramiento genético para encontrar resistencia al pasador del fruto, Neoleucinodes elegantalis, en tomate, Solanum lycopersicum L. se han basado en métodos tradicionales de introgresión genética por medio de retrocruzamiento. Sin embargo este proceso es largo y dispendioso. Debido a esta problemática, se utilizó la técnica molecular cDNA-AFLP para identificar genes involucrados en la resistencia a esta plaga en poblaciones segregantes de tomate, con el fin de identificar marcadores moleculares que puedan ser utilizados en futuros programas de fitomejoramiento. Los materiales de tomate evaluados fueron Unapal-Maravilla (susceptible), S. habrochaites var. glabratum, accesión PI 134418 (resistente) y tres retrocruzamientos obtenidos del cruzamiento interespecífico entre ambas especies. De estos materiales vegetales se extrajo el RNA total y se sintetizó el cDNA, el cual se utilizó como material de inicio de la técnica cDNA-AFLP. A partir del preamplificado de cDNA se evaluaron diferentes combinaciones de primers: +1+1, +3+3 y sus derivados. La combinación que presentó mejores amplificados fue la +1+1, para un total de 37 bandas polimórficas. De estas bandas, 9 fragmentos tipo EST (Expressed Sequence Target) fueron recuperados, siendo candidatos a explicar la resistencia al pasador del fruto en tomate.Breeding strategies for resistance to fruit borer Neoleucinodes elegantalis in tomato Solanum lycopersicum K L. have relied on traditional methods through genetic introgression by backcrossing. However, this process is slow and expensive. The cDNA-AFLP molecular technique was used to identify genes involved on fruit borer resistance in tomato segregating populations, The evaluated materials of tomato were: Unapal maravilla (susceptible to fruit borer) , S. habrochaites var. glabratum, acc. PI 134418 (resistant to fruit borer), and three interspecific backcross produced by crossbreeding between those two species. From these plant materials, total RNA was extracted and cDNA-AFLP technique was implemented. From the preamplified cDNA, different combinations of primers: +1+1, +3+3 and their derivates were evaluated. The combination that showed better amplified was +1+1 for a total of 37 polymorphic bands and 9 fragments type Expressed Sequence Target was isolated, which could explain the resistance of tomato to fruit borer. Date A point or period of time associated with an event in the lifecycle of the resource 2010 Subject The topic of the resource Neoleucinodes elegantalis, Solanum lycopersicum, cDNA-AFLP Source A related resource from which the described resource is derived Acta Agronómica Publisher An entity responsible for making the resource available Universidad Nacional de Colombia Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Agriculture Relation A related resource <a href="http://www.scielo.org.co/scielo.php?script=sci_arttext&amp;pid=S0120-28122010000300001" target="_blank" rel="noreferrer noopener">http://www.scielo.org.co/scielo.php?script=sci_arttext&amp;pid=S0120-28122010000300001</a>