https://socictopen.socict.org/files/original/46189668e70b969831416cd2af0c4e5a.pdf 99449b3d8fb00b48f4403da37e8c1b51 Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Coronavirus Description An account of the resource Dominio científico: Coronavirus Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Site-Specific Cleavage of RNAs Derived from the <i>PIM1</i> 3′-UTR by a Metal-Free Artificial Ribonuclease Creator An entity primarily responsible for making the resource Felix Zellmann, Laura Thomas, Ute Scheffer, Roland K. Hartmann, Michael W. Göbel Description An account of the resource Oligonucleotide conjugates of tris(2-aminobenzimidazole) have been reported previously to cleave complementary RNA strands with high levels of sequence and site specificity. The RNA substrates used in these studies were oligonucleotides not longer than 29-mers. Here we show that ~150&#8315;400-mer model transcripts derived from the 3&#8242;-untranslated region of the PIM1 mRNA reacted with rates and specificities comparable to those of short oligonucleotide substrates. The replacement of DNA by DNA/LNA mixmers further increased the cleavage rate. Tris(2-aminobenzimidazoles) were designed to interact with phosphates and phosphate esters. A cell, however, contains large amounts of phosphorylated species that may cause competitive inhibition of RNA cleavage. It is thus important to note that no loss in reaction rates was observed in phosphate buffer. This opens the way to in-cell applications for this type of artificial nuclease. Furthermore, we disclose a new synthetic method giving access to tris(2-aminobenzimidazoles) in multigram amounts. Date A point or period of time associated with an event in the lifecycle of the resource 2019 Subject The topic of the resource 2-aminobenzimidazole, cleavage of large RNA molecules, cleavage site selection, DNA/LNA mixmers, dye labeling, guanidine analogs, oligonucleotides, specificity of cleavage Identifier An unambiguous reference to the resource within a given context DOI: 10.3390/molecules24040807 Source A related resource from which the described resource is derived Molecules Publisher An entity responsible for making the resource available MDPI AG Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Organic chemistry Language A language of the resource EN