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            <name>Title</name>
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                <text>Agricultura sostenible</text>
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                <text>Dominio científico: Agricultura sostenible</text>
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          <name>Title</name>
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              <text>Assessment of Genetic Diversity and Structure of Large Garlic (Allium sativum) Germplasm Bank, by Diversity Arrays Technology “Genotyping-by-Sequencing” Platform (DArTseq)</text>
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              <text>Leticia A. Egea, Leticia A. Egea, Rosa Mérida-García, Andrzej Kilian, Pilar Hernandez, Gabriel Dorado</text>
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              <text>Garlic (Allium sativum) is used worldwide in cooking and industry, including pharmacology/medicine and cosmetics, for its interesting properties. Identifying redundancies in germplasm blanks to generate core collections is a major concern, mostly in large stocks, in order to reduce space and maintenance costs. Yet, similar appearance and phenotypic plasticity of garlic varieties hinder their morphological classification. Molecular studies are challenging, due to the large and expected complex genome of this species, with asexual reproduction. Classical molecular markers, like isozymes, RAPD, SSR, or AFLP, are not convenient to generate germplasm core-collections for this species. The recent emergence of high-throughput genotyping-by-sequencing (GBS) approaches, like DArTseq, allow to overcome such limitations to characterize and protect genetic diversity. Therefore, such technology was used in this work to: (i) assess genetic diversity and structure of a large garlic-germplasm bank (417 accessions); (ii) create a core collection; (iii) relate genotype to agronomical features; and (iv) describe a cost-effective method to manage genetic diversity in garlic-germplasm banks. Hierarchical-cluster analysis, principal-coordinates analysis and STRUCTURE showed general consistency, generating three main garlic-groups, mostly determined by variety and geographical origin. In addition, high-resolution genotyping identified 286 unique and 131 redundant accessions, used to select a reduced size germplasm-bank core collection. This demonstrates that DArTseq is a cost-effective method to analyze species with large and expected complex genomes, like garlic. To the best of our knowledge, this is the first report of high-throughput genotyping of a large garlic germplasm. This is particularly interesting for garlic adaptation and improvement, to fight biotic and abiotic stresses, in the current context of climate change and global warming.</text>
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              <text>2017</text>
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              <text>Breeding, DNA fingerprinting, Somatic mutation, phenotype, second-generation sequencing (SGS), third-generation sequencing (TGS)</text>
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          <name>Identifier</name>
          <description>An unambiguous reference to the resource within a given context</description>
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              <text>10.3389/fgene.2017.00098</text>
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          <name>Source</name>
          <description>A related resource from which the described resource is derived</description>
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              <text>Frontiers in Genetics</text>
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          <name>Publisher</name>
          <description>An entity responsible for making the resource available</description>
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              <text>Frontiers Media S.A.</text>
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          <name>Coverage</name>
          <description>The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant</description>
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              <text>Genetics</text>
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          <description>A related resource</description>
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              <text>&lt;a href="http://journal.frontiersin.org/article/10.3389/fgene.2017.00098/full" target="_blank" rel="noreferrer noopener"&gt;http://journal.frontiersin.org/article/10.3389/fgene.2017.00098/full&lt;/a&gt;</text>
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