https://socictopen.socict.org/files/original/70be0ca55b351c2e9fbfada16b574f31.pdf a0cbea94d0912b945e84406e9a2fae8b Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Coronavirus Description An account of the resource Dominio científico: Coronavirus Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Genotyping and phylogenetic analysis of canine parvovirus circulating in Egypt Creator An entity primarily responsible for making the resource Kawther Sayed Zaher, Wahid Hussein El-Dabae, Mostafa Mohamed El-Sebelgy, Naglaa Ibrahim Aly, Zeinab Taha Salama Description An account of the resource Aim: This study aimed to detect and characterize current genotypes of canine parvovirus (CPV) in Egypt during 2018.Materials and Methods: A total of 50 fecal swabs were collected from clinically infected domestic dogs of 2-5 months of age, suspected to suffer from CPV infection, from Cairo and Giza Governorates. The samples were subjected to qualitative antigen detection using the rapid test, followed by isolation on Madin-Darby Canine Kidney (MDCK) cells, molecular characterization with partial amplification of VP2 gene using polymerase chain reaction (PCR), followed by sequencing and phylogenetic analysis.Results: Out of 50 fecal samples, 20 samples were positive (40%) by Rapid CPV/canine coronavirus Ag Test Kit. These positive samples were cultured successfully on MDCK cells. Nine randomly chosen samples out of 30 apparently negative samples were amplified using PCR with primers Hfor and Hrev to yield a typical 630 bp fragment. Then, six randomly chosen samples out of nine were amplified using PCR with primers Pbs and Pbas to yield a typical 427 bp fragment. Sequencing, BLAST analysis and assembly of the two fragments (630 bp and 427 bp) to produce 912 bp fragments, in the six samples, revealed two serotypes CPV-2b and CPV-2c. The obtained strains were submitted to GenBank and given accession numbers MK642272, MK642273, MK642274, MK642275, MK642276, and MK642277. Phylogenetic analysis of the Egyptian strains serotype 2b illustrated that they were closely related to Thailand strains (accession numbers KP715709, KP715694, KP715701, and KP715700); while Egyptian strains serotype 2c was closely related to Thailand strains (accession numbers MH711894 and MH711902), Taiwanese strain (KU244254), Chinese strain (MF467242), and Vietnamese strain (accession number LC216910).Conclusion: The current research recommends further epidemiological studies to assess the extent of the occurrence of different serotypes of CPV in Egypt and the efficiency of imported and locally produced vaccines in protection against CPV infection. Date A point or period of time associated with an event in the lifecycle of the resource 2020 Subject The topic of the resource canine parvovirus, Egypt, genotyping, Phylogenetic analysis, serotyping Identifier An unambiguous reference to the resource within a given context DOI: 10.14202/vetworld.2020.326-333 Source A related resource from which the described resource is derived Veterinary World Publisher An entity responsible for making the resource available Veterinary World Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Veterinary medicine, Animal culture Language A language of the resource EN