https://socictopen.socict.org/files/original/908d5e39aedacbb27dcffabd406f02d6.pdf 3bc15847e617ebcb10933f5688d1b300 Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Agricultura sostenible Description An account of the resource Dominio científico: Agricultura sostenible Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Expression of a Deschampsia antarctica Desv. Polypeptide with Lipase Activity in a Pichia pastoris Vector Creator An entity primarily responsible for making the resource Claudia Rabert, Ana Gutiérrez-Moraga, Alejandro Navarrete, Darío Navarrete-Campos, León Bravo, Manuel Gidekel Description An account of the resource The current study isolated and characterized the Lip3F9 polypeptide sequence of Deschampsia antarctica Desv. (GeneBank Accession Number JX846628), which was found to be comprised of 291 base pairs and was, moreover, expressed in Pichia pastoris X-33 cells. The enzyme was secreted after 24 h of P. pastoris culture incubation and through induction with methanol. The expressed protein showed maximum lipase activity (35 U/L) with an optimal temperature of 37 °C. The lipase-expressed enzyme lost 50% of its specific activity at 25 °C, a behavior characteristic of a psychrotolerant enzyme. Recombinant enzyme activity was measured in the presence of ionic and non-ionic detergents, and a decrease in enzyme activity was detected for all concentrations of ionic and non-ionic detergents assessed. Date A point or period of time associated with an event in the lifecycle of the resource 2014 Subject The topic of the resource Deschampsia antarctica, Pichia pastoris, detergent tolerance, lipase Identifier An unambiguous reference to the resource within a given context 10.3390/ijms15022359 Source A related resource from which the described resource is derived International Journal of Molecular Sciences Publisher An entity responsible for making the resource available MDPI AG Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Biology (General), Chemistry Relation A related resource <a href="http://www.mdpi.com/1422-0067/15/2/2359" target="_blank" rel="noreferrer noopener">http://www.mdpi.com/1422-0067/15/2/2359</a>