https://socictopen.socict.org/files/original/2b8cc39841b7a8670480169bac6f74c9.pdf 2a959967a3b0864b1868f7b122e2917e Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Agricultura sostenible Description An account of the resource Dominio científico: Agricultura sostenible Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Resistance to 16-Membered Macrolides, Tiamulin and Lincomycin in a Swine Isolate of <i>Acholeplasma laidlawii</i> Creator An entity primarily responsible for making the resource María M. Tavío, Ana S. Ramírez, Carlos Poveda, Rubén S. Rosales, Cristina F. Malla, José B. Poveda Description An account of the resource Acholeplasma (A.) laidlawii is an opportunistic pathogen with the ability to disseminate resistance determinants to antibiotics; however, its resistance to macrolides has been less studied. The aim of the present study was to characterize the mechanisms responsible for the resistance to macrolides, tiamulin and lincomycin found in a strain of A. laidlawii isolated from a pig with pneumonia. MICs of erythromycin, 15- and 16-membered macrolides, tiamulin and lincomycin were determined by microdilution method with and without reserpine, an inhibitor of ABC efflux pumps and regions of the genome were sequenced. Reserpine only decreased lincomycin MIC but it did not change the MICs of macrolides and tiamulin. The analysis of the DNA sequence of 23S rRNA showed nucleotide substitutions at eight different positions, although none of them were at positions previously related to macrolide resistance. Five mutations were found in the L22 protein, one of them at the stop codon. In addition, two mutations were found in the amino acid sequence of L4. The combination of multiple mutations in the ribosomal proteins L22 and L4 together with substitutions in 23S rRNA DNA sequence was associated with the resistance to macrolides, the pleuromutilin and lincomycin in the studied A. laidlawii strain. Date A point or period of time associated with an event in the lifecycle of the resource 2021 Subject The topic of the resource <i>Acholeplasma laidlawii</i>, Lincomycin, Macrolide, antimicrobial resistance, tiamulin Identifier An unambiguous reference to the resource within a given context 10.3390/antibiotics10111415 Source A related resource from which the described resource is derived Antibiotics Publisher An entity responsible for making the resource available MDPI AG Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Therapeutics. Pharmacology Relation A related resource <a href="https://www.mdpi.com/2079-6382/10/11/1415" target="_blank" rel="noreferrer noopener">https://www.mdpi.com/2079-6382/10/11/1415</a>