https://socictopen.socict.org/files/original/71a4376b18079ab7df93e67cf134299f.pdf 857677d6b5e87fd3b56b1e4a6861f219 Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Agricultura sostenible Description An account of the resource Dominio científico: Agricultura sostenible Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Detection and identification of TMV infecting tomato under protected cultivation in Paraná State Creator An entity primarily responsible for making the resource Rodrigo Martins da Silva, Eliezer Rodrigues de Souto, Júlio Cézar Pedroso, Ricardo Arakava, Álvaro Manuel Rodrigues Almeida, Antônio Augusto Lazarini Barboza, João Batista Vida Description An account of the resource During an inspection in plastic houses in Sapopema, Paraná, 90% of tomato plants showed leaf abnormalities, probably associated with herbicide toxity. However, virus like symptoms developed in selected hosts after mechanical inoculatation. RT-PCR reactions using primers for an internal region within the movement protein gene of TMV and ToMV resulted in the amplification of a 409 bp cDNA fragment only by TMV primers. Deduced amino acids showed 100% identity when compared to TMV movement protein and 94% with ToMV. The RT-PCR protocol was efficient for quick and conclusive determination of virus species. The virus was purified and a polyclonal antiserum was raised for future surveys in tomato crops of Paraná. The partial genomic sequence obtained for TMV-Sapopema has been deposited under the accession number DQ173945, which is the first partial genomic sequence of an isolate of TMV from Brazil in the GenBank, and the first tomato virus isolate from Paraná to have some of its biological and molecular properties determined.Durante uma inspeção em cultivos protegidos de tomate em Sapopema, Paraná, foram observadas anormalidades foliares em 90% das plantas, indicando possivelmente a existência de um problema de fitotoxidade causada por herbicidas. Todavia, os sintomas manifestados nas hospedeiras após os ensaios de inoculação mecânica revelaram que os sintomas estariam relacionados a uma infecção por Tobamovirus. As reações de RT-PCR com oligonucleotídeos específicos para uma região interna da proteína de movimento de dois vírus comuns em tomate, TMV e ToMV, resultaram na amplificação de um fragmento de 409 pares de bases, apenas com os oligonucleotídeos específicos para o TMV. Após o sequenciamento, os aminoácidos deduzidos apresentaram identidade de 100% quando comparados com as seqüências das proteínas de movimento de outros isolados do TMV, e 94% de identidade com seqüências do ToMV. A RT-PCR demonstrou ser um método eficiente para a rápida e conclusiva determinação da espécie viral envolvida na infecção do tomateiro. A seqüência parcial do genoma do isolado de TMV de Sapopema, está depositada no GenBank sob o número de acesso DQ173945, sendo esta a primeira seqüência genômica parcial de um isolado de TMV do Brasil, e conforme o nosso conhecimento, o primeiro isolado de TMV do Paraná a ter algumas de suas propriedades biológicas e moleculares determinadas. Um anti-soro policlonal foi produzido, o que permitirá futuros levantamentos da ocorrência de Tobamovirus nas principais áreas de cultivo de tomate do Paraná. Date A point or period of time associated with an event in the lifecycle of the resource 2008 Subject The topic of the resource P30 protein, TMV, plasticulture Identifier An unambiguous reference to the resource within a given context 10.1590/S1516-89132008000500005 Source A related resource from which the described resource is derived Brazilian Archives of Biology and Technology Publisher An entity responsible for making the resource available Instituto de Tecnologia do Paraná (Tecpar) Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Biotechnology Relation A related resource <a href="http://www.scielo.br/scielo.php?script=sci_arttext&amp;pid=S1516-89132008000500005" target="_blank" rel="noreferrer noopener">http://www.scielo.br/scielo.php?script=sci_arttext&amp;pid=S1516-89132008000500005</a>