https://socictopen.socict.org/files/original/61b85747a3b039c607bce0d125230a88.pdf beee37fca84d6133fb0f796810d8a1f1 Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Agricultura sostenible Description An account of the resource Dominio científico: Agricultura sostenible Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Isolamento de Gluconacetobacter spp. em diferentes tipos de solos Isolation of Gluconacetobacter spp. in different soil types Creator An entity primarily responsible for making the resource Veronica Massena Reis, José Ivo Baldani, Carla Cristiane Rocha dos Santos, Liamara Perin Description An account of the resource O objetivo deste trabalho foi o aprimoramento da técnica de imunocaptura para utilização em amostras de solo contendo altos teores de argila e sua aplicação no isolamento de estirpes de Gluconacetobacter diazotrophicus a partir de amostras de solo cultivado com cana-de-açúcar e café. A técnica de imunocaptura foi aplicada com sucesso no isolamento de bactérias de amostras de solo. A modificação do método de imunocaptura com Al2(SO4)3 permitiu a sua aplicação em amostras de solo argiloso pela floculação da argila em suspensão. Este método mostrou-se efetivo no isolamento de G. diazotrophicus inoculada em amostras de solo arenoso e argiloso aos cinco dias após a inoculação. A sensibilidade máxima do método em isolar células de G. diazotrophicus mediante cultura pura foi de 10³ células mL-1 . A modificação da técnica permitiu o isolamento de Gluconacetobacter spp. de amostras de solo colhidas a 50 cm das raízes de plantas de café, mas não de amostras de solo colhidas à mesma distância de plantas de cana-de-açúcar.The objective of this work was to improve the immunocapture method for the application to soil samples containing high levels of clay particles and to utilize it for the isolation of Gluconacetobacter diazotrophicus strains from soil cultivated with sugarcane and coffee. The immunocapture technique was applied successfully for isolation of bacterium from soil samples. The modified immunocapture technique with Al2(SO4)3 allowed the application of the method using clayed soil samples by the flocculation of the suspended clay. It proved to be effective for isolation of G. diazotrophicus inoculated in sterile sandy and clay soils five days after inoculation. The maximum sensitivity of the method to isolate G. diazotrophicus cells, using pure culture, was 10³ cells mL-1. The modification of the technique allows the capture of Gluconacetobacter spp. from soil samples near the coffee plants but not from sugarcane soils. Date A point or period of time associated with an event in the lifecycle of the resource 2006 Subject The topic of the resource Bactéria diazotrófica, Immunology, Imunologia, café, cana-de-açúcar, coffee, diazotrophic bacterium, ecology, ecología, sugarcane Identifier An unambiguous reference to the resource within a given context 10.1590/S0100-204X2006000100012 Source A related resource from which the described resource is derived Pesquisa Agropecuária Brasileira Publisher An entity responsible for making the resource available Embrapa Informação Tecnológica Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Agriculture (General) Relation A related resource <a href="http://www.scielo.br/scielo.php?script=sci_arttext&amp;pid=S0100-204X2006000100012" target="_blank" rel="noreferrer noopener">http://www.scielo.br/scielo.php?script=sci_arttext&amp;pid=S0100-204X2006000100012</a>