SARS–associated Coronavirus Replication in Cell Lines
subject: research, SARS-CoV, Cell susceptibility, cytopathic effects
description: Given the potential for laboratory-associated severe acute respiratory syndrome–associated coronavirus (SARS-CoV) infections, we must know which cell lines are susceptible to the virus. We investigated 21 cell lines routinely used for virus isolation or research. After infection with SARS-CoV, cells were observed for cytopathic effects, and quantitative real-time polymerase chain reaction was used to measure ongoing viral replication. An indirect immunofluorescence assay was also used as a confirmatory test. The study identified 10 new cell lines capable of supporting the replication of SARS-CoV and confirmed the susceptibility of 4 cell lines previously reported. This study shows that SARS-CoV can be isolated in several cell lines commonly used for diagnostic or research purposes. It also shows that SARS-CoV can achieve high titers in several cell lines, sometimes in the absence of specific cytopathic effects.
creator: Thomas Tran, Mike Catton, Doris Chibo, Chris Birch, Julian Druce, Renata Kostecki, Matthew Kaye, Jessica Morris
source: Emerging Infectious Diseases
publisher: Centers for Disease Control and Prevention
date: 2006
identifier: DOI: 10.3201/eid1201.050496
coverage: Infectious and parasitic diseases, Medicine