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                <text>Coronavirus</text>
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                <text>Dominio científico: Coronavirus</text>
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              <text>Epidemiology and clinical profile of pathogens responsible for the hospitalization of children in Sousse area, Tunisia.</text>
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              <text>Ines Brini, Aida Guerrero, Naila Hannachi, Jihene Bouguila, Dorothea Orth-Höller, Amira Bouhlel, Lamia Boughamoura, Benjamin Hetzer, Wegene Borena, Britta Schiela, Dorothee von Laer, Jalel Boukadida, Heribert Stoiber</text>
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              <text>This study aimed to identify a broad spectrum of respiratory pathogens from hospitalized and not-preselected children with acute respiratory tract infections in the Farhat Hached University-hospital of Sousse, Tunisia. Between September 2013 and December 2014, samples from 372 children aged between 1 month and 5 years were collected, and tested using multiplex real-time RT-PCR by a commercial assay for 21 respiratory pathogens. In addition, samples were screened for the presence of Streptococcus pneumoniae 16S rDNA using real-time PCR. The viral distribution and its association with clinical symptoms were statistically analyzed. Viral pathogens were detected in 342 (91.93%) of the samples of which 28.76% were single positive and 63.17% had multiple infections. The most frequent detected viruses were rhinovirus (55.64%), respiratory syncytial virus A/B (33.06%), adenovirus (25.00%), coronavirus NL63, HKU1, OC43, and 229E (21.50%), and metapneumovirus A/B (16.12%). Children in the youngest age group (1-3 months) exhibited the highest frequencies of infection. Related to their frequency of detection, RSV A/B was the most associated pathogen with patient's demographic situation and clinical manifestations (p</text>
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              <text>2017</text>
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              <text>DOI: 10.1371/journal.pone.0188325</text>
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              <text>PLoS ONE</text>
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              <text>Public Library of Science (PLoS)</text>
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              <text>Science, Medicine</text>
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