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                <text>Dominio científico: Coronavirus</text>
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              <text>Emilie Frobert, Antonin Bal, Valérie Cheynet, Guy Oriol, Florence Morfin, Karen Brengel-Pesce, Laurence Generenaz, Gregory Destras, Geneviève Billaud, Sibyle Etievant, Vanessa Escuret, Maude Bouscambert, Laurence Josset, Alexandre Gaymard</text>
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              <text>A reliable diagnostic assay is crucial to early detect new COVID-19 cases and limit severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) transmission. Since the onset of the COVID-19 pandemic, the World Health Organization has published several diagnostic molecular approaches developed by referral laboratories, including Charité (Germany), HKU (Hong Kong), China CDC (China), US CDC (United States), and Institut Pasteur, Paris (France). We aimed to compare the sensitivity and specificity of these different RT-PCR assays using SARS-CoV-2 cell culture supernatants and clinical respiratory samples. Overall, the different RT-PCR assays performed well for SARS-CoV-2 detection and were all specific except the N Charité (Germany), and N2 US CDC (United States) assays. RdRp Institut Pasteur (IP2, IP4), N China CDC, and N1 US CDC were found to be the most sensitive assays. The data presented herein are of prime importance to facilitate the equipment choice of diagnostic laboratories, as well as for the development of marketed tests.</text>
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              <text>DOI: 10.3390/jcm9061871</text>
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              <text>Journal of Clinical Medicine</text>
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              <text>MDPI AG</text>
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