Novel rapid identification of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) by real-time RT-PCR using BD Max Open System in Taiwan
subject: Real-time RT-PCR, SARS-CoV-2, BD Max platform
description: Coronavirus disease 2019 has become a worldwide pandemic. By April 7, 2020, approximately 1,279,722 confirmed cases were reported worldwide including those in Asia, European Region, African Region and Region of the Americas. Rapid and accurate detection of Severe Acute Respiratory Syndrome Virus 2 (SARS-CoV-2) is critical for patient care and implementing public health measures to control the spread of infection. In this study, we developed and validated a rapid total nucleic acid extraction method based on real‐time RT-PCR for reliable, high‐throughput identification of SARS-CoV-2 using the BD MAX platform. For clinical validation, 300 throat swab and 100 sputum clinical samples were examined by both the BD MAX platform and in-house real-time RT-PCR methods, which showed 100% concordant results. This BD MAX protocol is fully automated and the turnaround time from sample to results is approximately 2.5 h for 24 samples compared to 4.8 h by in-house real-time RT-PCR. Our developed BD MAX RT-PCR assay can accurately identify SARS-CoV-2 infection and shorten the turnaround time to increase the effectiveness of control and prevention measures for this emerging infectious disease.
creator: Cherng-Lih Perng, Chien-Wen Chen, Feng-Yee Chang, Chih-Kai Chang, Shih-Hung Tsai, Kuo-Ming Yeh, Jung-Chung Lin, Sheng-Kang Chiu, Hung-Sheng Shang, Yihui Wang, Ming-Jr Jian, Hsing-Yi Chung, Shu-Jung Liao, Shih-Yi Li, Shan-Shan Hsieh
source: PeerJ
publisher: PeerJ Inc.
date: 2020
identifier: DOI: 10.7717/peerj.9318
coverage: Medicine