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                <text>Dominio científico: Coronavirus</text>
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              <text>Differentiation of Bovine coronavirus (BCoV) genotypes by a restriction enzyme assay</text>
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              <text>Sibele Pinheiro de Souza, Karen Miyuki Asano, Thaisa Lucas Sandri, Iracema Nunes de Barros, Leonardo José Richtzenhain, Paulo Eduardo Brandão</text>
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              <text>This article reports the use of the GsuI restriction enzyme to differentiate genotypes of Bovine Coronavirus (BCoV), based on an 18-nucleotide deletion of S1-coding region found in one of the two genotypes. It was concluded that this assay can be used as a rapid tool for BCoV genotypes differentiation.</text>
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              <text>Bovine coronavirus, restriction enzyme, genotypes, Genes</text>
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              <text>DOI: 10.1590/S1517-83822010000300034</text>
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              <text>Brazilian Journal of Microbiology</text>
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              <text>Sociedade Brasileira de Microbiologia</text>
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              <text>Microbiology</text>
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              <text>EN</text>
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