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            <name>Title</name>
            <description>A name given to the resource</description>
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                <text>Coronavirus</text>
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            <name>Description</name>
            <description>An account of the resource</description>
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                <text>Dominio científico: Coronavirus</text>
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          <name>Title</name>
          <description>A name given to the resource</description>
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              <text>Sobre a evolução do vírus da bronquite infecciosa das galinhas em células VERO</text>
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          <name>Creator</name>
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              <text>Paulo Eduardo Brandão, Leonardo José Richtzenhain, Laura Yaneth Villarreal Buitrago</text>
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          <name>Description</name>
          <description>An account of the resource</description>
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              <text>O vírus da bronquite infecciosa das galinhas (IBV) (Nidovirales: Coronaviridae) é um Gammacoronavirus com a maior taxa evolutiva no gênero e, apesar de uma recentemente relatada atividade corretiva de sua polimerase, a diversidade intra e inter-hospedeiros é um fenômeno bem documentado. Este estudo objetivou avaliar a variação genética após passagens seriais de uma amostra de IBV variante. A amostra CRG-BETA, propagada em embriões de galinhas, foi inoculada em monocamadas de células VERO até a quarta passagem e cada passagem foi monitorada com uma RTPCR para a região S1 do gene S (nt 705 a 1094) e uma RT-PCR para o mRNA da proteína 5a do vírus. Todas as passagens foram positivas para S1 e as passagens 1 a 3 para mRNA 5a; sequências de S1 não apresentaram polimorfismos. O encontro de mRNA de IBV nos cultivos celulares demonstra que a amostra CRG-BETA está replicando nas células VERO e, em relação à análise de S1, a ausência de mutações de nucleotídeos demonstra que a amostra CRG-BETA pode ter atingido um estado fixo. Como conclusão, diferentes genótipos de IBV apresentam diferentes padrões evolutivos não apenas in vivo, como previamente conhecido, mas também in vitro, como aqui relatado.</text>
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          <name>Date</name>
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              <text>2012</text>
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          <name>Subject</name>
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              <text>IBV, Espícula, evolução, in vitro</text>
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          <name>Identifier</name>
          <description>An unambiguous reference to the resource within a given context</description>
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              <text>DOI: 10.11606/issn.2318-3659.v49i5p386-390</text>
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          <name>Source</name>
          <description>A related resource from which the described resource is derived</description>
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              <text>Brazilian Journal of Veterinary Research and Animal Science</text>
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        <element elementId="45">
          <name>Publisher</name>
          <description>An entity responsible for making the resource available</description>
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              <text>Universidade de São Paulo</text>
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          <name>Coverage</name>
          <description>The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant</description>
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              <text>Animal culture</text>
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          <name>Language</name>
          <description>A language of the resource</description>
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              <text>EN, PT</text>
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