Immunofluorescence Assay for Serologic Diagnosis of SARS

Immunofluorescence Assay for Serologic Diagnosis of SARS

Paul K. S. Chan, King-Cheung Ng, Rickjason C. W. Chan, Rebecca K. Y. Lam, Viola C. Y. Chow, Mamie Hui, Alan Wu, Nelson Lee, Florence H.Y. Yap, Frankie W. T. Cheng, Joseph J. Y. Sung, John S. Tam

We evaluated a virus-infected cell-based indirect immunofluorescence assay for detecting anti–severe acute respiratory syndrome-associated coronavirus (SARS-CoV) immunoglobulin (Ig) G antibody. All confirmed SARS cases demonstrated seroconversion or fourfold rise in IgG antibody titer; no control was positive. Sensitivity and specificity of this assay were both 100%. Immunofluorescence assay can ascertain the status of SARS-CoV infection.

2004

coronavirus, diagnosis, serology, Sensitivity, specificity, severe acute respiratory syndrome

DOI: 10.3201/eid1003.030493

Emerging Infectious Diseases

Centers for Disease Control and Prevention

Infectious and parasitic diseases, Medicine

EN