https://socictopen.socict.org/files/original/9e40533d849b517b62908242533d53bb.pdf 498a36cf47ebae2854f6d6516b96fa9f Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Coronavirus Description An account of the resource Dominio científico: Coronavirus Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Optimisation of qualitative and semi-quantitative detection of genetically modified crops by PCR Creator An entity primarily responsible for making the resource Tomáš Vyhnánek, Pavel Hanáček Description An account of the resource For qualitative and semi-quantitative detection of genetically modified crops we selected the detection of the frequently used promoter 35S CaMV. To optimise the method we used two commercially available genotypes of maize from the company Monsanto (USA), i.e. the transgenic hybrid Bt-maize line MON810 and a genetically non-modified control (isogenic line to MON810). We tested the pri­mers and PCR programmes described by Greiner et al. (2005) and Hernandéz et al. (2005). When applying PCR methods of detection of Bt-maize the first step was to optimise the protocol for the detection of the maize genome and detection of the specific sites of genetically modified MON810 maize. For detection of the maize genome we selected the primers IVR1-F and IVR1-R (invertase gene) which verify the presence of the maize genome by a 226 bp product. For qualitative detection of the insert of Bt-maize MON810 the primer pairs VW01/VW03 (Greiner et al., 2005) and BT03/BT04 (Hernandéz et al., 2005) were used to detect the 35S CaMV promoter. Products of the size 178 bp and 280 bp, respectively, verify its presence. Based on the results of qualitative PCR we selected the primers VW01/VW03 for semi-quantitative detection of the amount of DNA of Bt-maize. For semi-quantitative PCR we have chosen sampling of the amplification product in the 30th cycle of the PCR reaction. In the genetically unmodified control a detection limit of 1% of admixture of Bt-maize was determined when using semi-quantitative PCR. The same primers as for semi-quantitative PCR were also used for multiplex PCR but with half the concentration of primers for standard PCR. This protocol however will have to be further optimised. The presented results introduce PCR methods for qualitative and semi-quantitative detection of DNA of the genetically modified Bt-maize MON810 which can also be used for other GM crops containing the 35S CaMV promoter. It could be suitable to use these methods for the qualitative detection and/or for screening analyses of the detection of successfulness of transformation experiments. Date A point or period of time associated with an event in the lifecycle of the resource 2009 Subject The topic of the resource PCR, qualitative detection, semi-quantitative detection, multiplex-PCR, Transgenic crops, maize MON810 Identifier An unambiguous reference to the resource within a given context DOI: 10.11118/actaun200957050313 Source A related resource from which the described resource is derived Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis Publisher An entity responsible for making the resource available Mendel University Press Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Biology (General), Agriculture Language A language of the resource EN