https://socictopen.socict.org/files/original/0bcbf3e94feea7daafb3fc3dabd81626.pdf c2d6679cdc77bc028ee30e516ca7c212 Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Coronavirus Description An account of the resource Dominio científico: Coronavirus Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Simultaneous detection and differentiation of canine parvovirus and feline parvovirus by high resolution melting analysis Creator An entity primarily responsible for making the resource Yaru Sun, Yuening Cheng, Peng Lin, Hewei Zhang, Li Yi, Mingwei Tong, Zhigang Cao, Shuang Li, Shipeng Cheng, Jianke Wang Description An account of the resource Abstract Background Canine parvovirus (CPV) and feline parvovirus (FPV) are causative agents of diarrhea in dogs and cats, which manifests as depression, vomiting, fever, loss of appetite, leucopenia, and diarrhea in young animals. CPV and FPV can single or mixed infect cats and cause disease. To diagnose sick animals effectively, an effective virus diagnostic and genome typing method with high sensitivity and specificity is required. Results In this study, a conserved segment containing one SNP A4408C of parvovirus was used for real-time PCR amplification. Subsequently, data were auto-analyzed and plotted using Applied Biosystems® High Resolution Melt Software v3.1. Results showed that CPV and FPV can be detected simultaneously in a single PCR reaction. No cross-reactions were observed with canine adenovirus, canine coronavirus, and canine distemper virus. The assay had a detection limit of 4.2 genome copies of CPV and FPV. A total of 80 clinical samples were subjected to this assay, as well as to conventional PCR-sequence assay and virus isolation. Results showed that the percentage of agreement of the assay and other methods are high. Conclusions In short, we have developed a diagnostic test for the accurate detection and differentiation of CPV and FPV in fecal samples, which is also cost effective. Date A point or period of time associated with an event in the lifecycle of the resource 2019 Subject The topic of the resource simultaneous detection, Differentiation, canine parvovirus, Feline parvovirus, HRM Identifier An unambiguous reference to the resource within a given context DOI: 10.1186/s12917-019-1898-5 Source A related resource from which the described resource is derived BMC Veterinary Research Publisher An entity responsible for making the resource available BMC Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Veterinary medicine Language A language of the resource EN