https://socictopen.socict.org/files/original/5f949d6337b988c27fe23a2abf56c7a3.pdf 4eed4ed197556f73cbedf15341cf0889 Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Coronavirus Description An account of the resource Dominio científico: Coronavirus Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Detection of respiratory viruses by real-time polymerase chain reaction in outpatients with acute respiratory infection Creator An entity primarily responsible for making the resource Ronaldo Bragança Martins Júnior, Sharon Carney, Daniel Goldemberg, Lucas Bonine, Liliana Cruz Spano, Marilda Siqueira, Rita Elizabeth Checon Description An account of the resource Viruses are the major contributors to the morbidity and mortality of upper and lower acute respiratory infections (ARIs) for all age groups. The aim of this study was to determine the frequencies for a large range of respiratory viruses using a sensitive molecular detection technique in specimens from outpatients of all ages with ARIs. Nasopharyngeal aspirates were obtained from 162 individuals between August 2007-August 2009. Twenty-three pathogenic respiratory agents, 18 respiratory viruses and five bacteria were investigated using multiplex real-time reverse transcriptase polymerase chain reaction (RT-PCR) and indirect immunofluorescence assay (IIF). Through IIF, 33 (20.4%) specimens with respiratory virus were recognised, with influenza virus representing over half of the positive samples. Through a multiplex real-time RT-PCR assay, 88 (54.3%) positive samples were detected; the most prevalent respiratory viral pathogens were influenza, human rhinovirus and respiratory syncytial virus (RSV). Six cases of viral co-detection were observed, mainly involving RSV. The use of multiplex real-time RT-PCR increased the viral detection by 33.9% and revealed a larger number of respiratory viruses implicated in ARI cases, including the most recently described respiratory viruses [human bocavirus, human metapneumovirus, influenza A (H1N1) pdm09 virus, human coronavirus (HCoV) NL63 and HCoV HKU1]. Date A point or period of time associated with an event in the lifecycle of the resource 2014 Subject The topic of the resource Respiratory Infections, real-time PCR, Multiplex, Viruses Identifier An unambiguous reference to the resource within a given context DOI: 10.1590/0074-0276140046 Source A related resource from which the described resource is derived Memórias do Instituto Oswaldo Cruz. Publisher An entity responsible for making the resource available Instituto Oswaldo Cruz, Ministério da Saúde Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Arctic medicine. Tropical medicine, Microbiology Language A language of the resource EN