https://socictopen.socict.org/files/original/909b0af5bb63923623b4af17d9be6fd7.pdf 21a26ea37ab87a97d9623974ee9900c8 Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Coronavirus Description An account of the resource Dominio científico: Coronavirus Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Ultrastructural Characterization of SARS Coronavirus Creator An entity primarily responsible for making the resource Cynthia S. Goldsmith, Kathleen M. Tatti, Thomas G. Ksiazek, Pierre E Rollin, James A. Comer, William W. Lee, Paul A. Rota, Bettina Bankamp, William J. Bellini, Sherif R. Zaki Description An account of the resource Severe acute respiratory syndrome (SARS) was first described during a 2002–2003 global outbreak of severe pneumonia associated with human deaths and person-to-person disease transmission. The etiologic agent was initially identified as a coronavirus by thin-section electron microscopic examination of a virus isolate. Virions were spherical, 78 nm in mean diameter, and composed of a helical nucleocapsid within an envelope with surface projections. Herein, we show that infection with the SARS-associated coronavirus resulted in distinct ultrastructural features: double-membrane vesicles, nucleocapsid inclusions, and large granular areas of cytoplasm. These three structures and the coronavirus particles were shown to be positive for viral proteins and RNA by using ultrastructural immunogold and in situ hybridization assays. In addition, ultrastructural examination of a bronchiolar lavage specimen from a SARS patient showed numerous coronavirus-infected cells with features similar to those in infected culture cells. Electron microscopic studies were critical in identifying the etiologic agent of the SARS outbreak and in guiding subsequent laboratory and epidemiologic investigations. Date A point or period of time associated with an event in the lifecycle of the resource 2004 Subject The topic of the resource severe acute respiratory syndrome, SARS virus, coronavirus, electron microscopy, immunogold techniques, in situ hybridization Identifier An unambiguous reference to the resource within a given context DOI: 10.3201/eid1002.030913 Source A related resource from which the described resource is derived Emerging Infectious Diseases Publisher An entity responsible for making the resource available Centers for Disease Control and Prevention Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Infectious and parasitic diseases, Medicine Language A language of the resource EN