1H, 13C, 15N and 31P chemical shift assignment for stem-loop 4 from the 5'-UTR of SARS-CoV-2.

1H, 13C, 15N and 31P chemical shift assignment for stem-loop 4 from the 5'-UTR of SARS-CoV-2.

Stephen A Peter, Nusrat S Qureshi, Julia E Weigand, Jennifer Vögele, Jan-Peter Ferner, Nadide Altincekic, Jasleen Kaur Bains, Betül Ceylan, Boris Fürtig, J Tassilo Grün, Martin Hengesbach, Katharina F Hohmann, Daniel Hymon, Bozana Knezic, Frank Löhr, Dennis Pyper, Christian Richter, Andreas Schlundt, Harald Schwalbe, Elke Stirnal, Alexey Sudakov, Anna Wacker, Julia Wirmer-Bartoschek, Jens Wöhnert, Elke Duchardt-Ferner

The SARS-CoV-2 virus is the cause of the respiratory disease COVID-19. As of today, therapeutic interventions in severe COVID-19 cases are still not available as no effective therapeutics have been developed so far. Despite the ongoing development of a number of effective vaccines, therapeutics to fight the disease once it has been contracted will still be required. Promising targets for the development of antiviral agents against SARS-CoV-2 can be found in the viral RNA genome. The 5'- and 3'-genomic ends of the 30 kb SCoV-2 genome are highly conserved among Betacoronaviruses and contain structured RNA elements involved in the translation and replication of the viral genome. The 40 nucleotides (nt) long highly conserved stem-loop 4 (5_SL4) is located within the 5'-untranslated region (5'-UTR) important for viral replication. 5_SL4 features an extended stem structure disrupted by several pyrimidine mismatches and is capped by a pentaloop. Here, we report extensive 1H, 13C, 15N and 31P resonance assignments of 5_SL4 as the basis for in-depth structural and ligand screening studies by solution NMR spectroscopy.

2021

SARS-CoV-2, Covid19-NMR, Solution NMR-spectroscopy, RNA genome, 5′-UTR, 5_SL4

10.1007/s12104-021-10026-7

Biomolecular NMR assignments