https://socictopen.socict.org/files/original/1e9d514f9d0d556660a2a17690c25f0d.pdf d3aa0277f9f7b4e8ac4d9e1b0a831b15 Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Coronavirus Description An account of the resource Dominio científico: Coronavirus Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource MERS-CoV Accessory ORFs Play Key Role for Infection and Pathogenesis Creator An entity primarily responsible for making the resource Vineet D. Menachery, Hugh D. Mitchell, Adam S. Cockrell, Lisa E Gralinski, Boyd L. Yount, Rachel L. Graham, Eileen T. McAnarney, Madeline G. Douglas, Trevor Scobey, Anne Beall, Kenneth Dinnon, Jacob F. Kocher, Andrew E. Hale, Kelly G. Stratton, Katrina M. Waters, Ralph S. Baric, Vincent R. Racaniello Description An account of the resource While dispensable for viral replication, coronavirus (CoV) accessory open reading frame (ORF) proteins often play critical roles during infection and pathogenesis. Utilizing a previously generated mutant, we demonstrate that the absence of all four Middle East respiratory syndrome CoV (MERS-CoV) accessory ORFs (deletion of ORF3, -4a, -4b, and -5 [dORF3-5]) has major implications for viral replication and pathogenesis. Importantly, attenuation of the dORF3-5 mutant is primarily driven by dysregulated host responses, including disrupted cell processes, augmented interferon (IFN) pathway activation, and robust inflammation. In vitro replication attenuation also extends to in vivo models, allowing use of dORF3-5 as a live attenuated vaccine platform. Finally, examination of ORF5 implicates a partial role in modulation of NF-κB-mediated inflammation. Together, the results demonstrate the importance of MERS-CoV accessory ORFs for pathogenesis and highlight them as potential targets for surveillance and therapeutic treatments moving forward. Date A point or period of time associated with an event in the lifecycle of the resource 2017 Identifier An unambiguous reference to the resource within a given context DOI: 10.1128/mBio.00665-17 Source A related resource from which the described resource is derived mBio Publisher An entity responsible for making the resource available American Society for Microbiology Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Microbiology Language A language of the resource EN