https://socictopen.socict.org/files/original/5ecacedd57d3c2b1a440b9122e5564c1.pdf acb43e450a5e6b1459f9404471daa52b Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Coronavirus Description An account of the resource Dominio científico: Coronavirus Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Gene Variations in <i>Cis</i>-Acting Elements between the Taiwan and Prototype Strains of Porcine Epidemic Diarrhea Virus Alter Viral Gene Expression Creator An entity primarily responsible for making the resource Tsung-Lin Tsai, Chen-Chang Su, Ching-Chi Hsieh, Chao-Nan Lin, Hui-Wen Chang, Chen-Yu Lo, Ching-Houng Lin, Hung-Yi Wu Description An account of the resource In 2013, the outbreak of porcine epidemic diarrhea (PED) in Taiwan caused serious economic losses. In this study, we examined whether the variations of the cis-acting elements between the porcine epidemic diarrhea virus (PEDV) Taiwan (TW) strain and the prototype strain CV777 alter gene expression. For this aim, we analyzed the variations of the cis-acting elements in the 5&#8242; and 3&#8242; untranslated regions (UTRs) between the PEDV TW, CV777, and other reference strains. We also determined the previously unidentified transcription regulatory sequence (TRS), a sequence motif required for coronavirus transcription, and found that a nucleotide deletion in the TW strain, in comparison with CV777 strain, immediately downstream of the leader core sequence alters the identity between the leader TRS and the body TRS. Functional analyses using coronavirus defective interfering (DI) RNA revealed that such variations in cis-acting elements for the TW strain compared with the CV777 strain have an influence on the efficiency of gene expression. The current data show for the first time the evolution of PEDV in terms of cis-acting elements and their effects on gene expression, and thus may contribute to our understanding of recent PED outbreaks worldwide. Date A point or period of time associated with an event in the lifecycle of the resource 2018 Subject The topic of the resource porcine epidemic diarrhea virus, <i>cis</i>-acting element, gene evolution, gene expression, nucleotide composition Identifier An unambiguous reference to the resource within a given context DOI: 10.3390/genes9120591 Source A related resource from which the described resource is derived Genes Publisher An entity responsible for making the resource available MDPI AG Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Genetics Language A language of the resource EN